恒磁场对大鼠骨髓间充质干细胞增殖的影响  

Effects of Constant Magnetic Fields on Mesenchymal Stem Cells of Rat

作　　者：徐巧玲[1] 罗二平[2] 路丽华[3] 李雪琦[2] 罗鹏[2] 申广浩[2] 

机构地区：[1]第四军医大学护理系,陕西西安710032 [2]第四军医大学生物医学工程系,陕西西安710032 [3]第四军医大学药学系,陕西西安710032

出　　处：《中国医学物理学杂志》2009年第1期999-1002,共4页Chinese Journal of Medical Physics

基　　金：国家自然科学基金资助项目(No.50377044);陕西省自然科学基金资助项目(No.2006C211)

摘　　要：目的:探讨中心磁场强度为0.2T的恒磁场对大鼠骨髓间充质干细胞的影响。方法:用全骨髓贴壁法分离大鼠骨髓间充质干细胞,对生长良好的第3代细胞进行中心磁场强度为0.2T的恒磁场刺激,MTT法测定细胞增殖水平,流式细胞仪法测定细胞周期变化。结果:大鼠骨髓间充质干细胞经磁场刺激5d后,实验组细胞增殖水平和(S+G2/M)期细胞数量均有不同程度提高,差异有统计学意义(P<0.05)。结论:恒磁场能促进体外培养大鼠骨髓间充质干细胞的增殖水平。Objective： To study the effects of constant magnetic fields with the on mesenchymal stem cells of rat. Methods： The mesenchymal stem cells were obtained by using whole marrow attachment method. The third generation cells were irradiated by using of constant magnetic fields, and the central intensity was 0.2T. MTT was employed to evaluate the levels of proliferation. The parameters of the cells cycle were detected with the flow cytometer （FCM）. Results： After 5 days of irradiation by magnetic fields, the proliferation of the mesenchymal stem cells in rat was accelerated and the percentage of cells at the （S＋G2/M） phase was increased significantly （P〈0.05）. Conclusion： Irradiation of constant magnetic fields could increase the proliferation of mesenchymal stem cells of rat in vitro.

关 键 词：恒磁场 骨髓间充质干细胞 增殖 

分 类 号：R644[医药卫生—外科学]