兔脊髓缺血再灌注损伤时凝血功能的变化及谷氨酰胺预处理的影响  

Coagulation functions in rabbits with spinal cord ischemia-reperfusion injury and effect of glutamine precondition

作　　者：巩守平[1] 王文涛[1] 钟大林[1] 吕健[1] 吴锋[2] 车金[2] 僧志远[1] 贺西京[3] 

机构地区：[1]西安交通大学医学院第二附属医院神经外科,陕西西安710004 [2]西安交通大学医学院血液病实验室,陕西西安710004 [3]西安交通大学医学院第二附属医院骨科,陕西西安710004

出　　处：《第四军医大学学报》2009年第3期251-253,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨兔脊髓缺血再灌注损伤(SCIRI)时凝血功能的变化、意义及谷氨酰胺预处理对其影响.方法:采用Zivin法建立SCIRI模型,动态观察SCIRI及谷氨酰胺预处理时血液PT(凝血酶原时间),APTT(部分凝血活酶时间),TT(凝血酶时间)和FIB(纤维蛋白原)的变化.结果:假手术组PT,APTT,FIB逐渐升高(P>0.05),TT逐渐降低至缺血45min时显著降低然后逐渐升高(P<0.01);缺血再灌注组(I/R)凝血四项各项指标逐渐升高,2 h达到最高峰后逐渐下降,12 h降到缺血前水平,在这一过程中,除PT外,45 min,2 h,6h时间点与缺血前比较均有显著差异(P<0.01);谷氨酰胺预处理组凝血4项各项指标与I/R组比较仅有下降趋势(P>0.05).结论:SCIRI引起凝血状态显著改变,可能为凝血因子水平、活性及功能改变导致,谷氨酰胺预处理没有改善SCI-RI引起凝血状态改变.AIM： To explore the changes of coagulation functions in rabbits with spinal cord isehemia-reperfusion injury （SCI- RI） and the effect of glutamine precondition. METHODS： SCI- RI model were established with Zivin method in rabbits. PT, APTT, TT and FIB were measured at different time point before and after injury. RESULTS： PT, APTT, TT and FIB of rabbits with SCIRI increased obviously, reached the peak value after reperfusion of 2 hours, then started to decrease and reached the level below pre-ischemia value at the 12th hour after reperfusion. In the process, except PT, the other 3 indices were significantly higher at 45 min, 2 and 6 h than those pre-isehemia （P 〈 0.01 ）. PT, APTT, TT and FIB of rabbits with SCIRI with glutamine precondition were lower than those without glutamine precondition, but with no statistically significant differences. CONCLUSION： SCIRI leads to significant changes of coagulation functions and glutamine precondition fail to improve them.

关 键 词：谷氨酰胺 脊髓 脊髓缺血再灌注 凝血 

分 类 号：R651.2[医药卫生—外科学]