一种猪-鼠B细胞杂交瘤的制备方法  

Preparation of a swine-mouse hybridoma

作　　者：李姣[1] 郭建英[2] 王爱萍[1] 邓瑞广[1] 张淑杰[1] 游雷鸣[1] 

机构地区：[1]郑州大学生物工程系,河南郑州450001 [2]第四军医大学西京医院,陕西西安710033

出　　处：《第四军医大学学报》2009年第3期275-278,共4页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30471287)

摘　　要：目的:探索一种异源杂交瘤细胞株建立的方法.方法:以CSFV分点肌肉注射健康猪,成功诱导机体产生抗CS-FV的抗体.取免疫猪脾脏与鼠骨髓瘤细胞NS0融合,经筛选与克隆以获得猪源抗CSFV的mAb,并测定mAb的中和性及其效价.结果:成功筛选到15株抗CSFV的猪源mAb,中和实验结果表明9株mAb具有中和性,且同样识别重组的E2蛋白.结论:猪源mAb的成功制备为揭示CSFV在猪体内的免疫机制奠定了基础.AIM： To search for a method to prepare a swinemouse hybridoma. METHODS： Healthy swines were immunized with classical swine fever virus （CSFV） by intramuscular injection to induce anti-CSFV antibody. B cells of the immunized swine were fused with mouse myeloma cells NS0 and swine-mouse hybridoma was selected by CSFV and E2 protein. Neutralization test was carried out to identify the neutralizing titers of the swine antibodies. RESULTS ： Fifteen strains of anti-CSFV swine-mouse monoclonal antibodies （mAb） were obtained after several times of screening and cloning, 9 of which were neutralizing antibodies and were recognized by recombinant E2 protein. CONCLUSION： The swine mAb may provide a valuable basis for further research of immunologic mechanism of CSFV in swines.

关 键 词：异源单克隆抗体 CSFV E2蛋白 原核表达 

分 类 号：R392.12[医药卫生—免疫学]