ox-LDL对巨噬细胞表达miRNA-155和miRNA-146a/b的影响  

Effect of ox-LDL on the expressions of miRNA-155 and miRNA-146a/b in macrophage

作　　者：姚瑞[1] 程翔[1] 张金盈[2] 廖玉华[1] 余娴[1] 唐婷婷[1] 聂少芳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,湖北武汉430022 [2]郑州大学第一附属医院心内科,河南郑州450052

出　　处：《河南医学研究》2008年第4期317-319,共3页Henan Medical Research

基　　金：国家重点基础研究发展计划(973计划No:2007CB512000)

摘　　要：目的:探讨氧化低密度脂蛋白(ox-LDL)刺激对巨噬细胞表达miRNA-155和miRNA-146a/b的影响。方法:培养人巨噬细胞,随机分为三组:空白对照组、ox-LDL(10μg/mL)组和ox-LDL(20μg/mL)组,刺激6h后,收集细胞,实时定量PCR方法检测miRNA-155和miRNA-146a/b的表达。结果:ox-LDL刺激可以增加巨噬细胞表达miRNA-155和miRNA-146a/b,且呈浓度依赖性。结论:miRNA-155和miRNA-146a/b可能参与ox-LDL致动脉粥样硬化过程。Objective： To investigate the effect of ox-LDL on the expression of miRNA-155 and miRNA-146a/b in macrophage. Methods： Human macrophages were cultured. And different concentrations of oxidative low-density lipoprotein （ox-LDL） （0, 10 μg/mL and 20 μg/mL） were used to stimulate the macrophages respectively. And then real-time RT-PCR was used to detected the expressions of miRNA-155 and miRNA-146a/b. Results： ox-LDL stimulation could increase the expressions of miRNA-155 and miRNA-146a/b in macrophage. And the increasing was positively related to the concentration of ox-LDL. Conclusion ： miRNA-155 and miRNA-146a/b might take part in the pro-atherosclerosis process by ox-LDL.

关 键 词：氧化低密度脂蛋白 miRNA-155 miRNA-146a/b 巨噬细胞 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]