聚氨酯/多壁碳纳米管复合材料电纺丝支架对成纤维细胞生长的促进  

Enhancement of electrospun scaffold of polyurethane/carbon nanotubes composite on fibroblasts growth

作　　者：孟洁[1] 孔桦[2] 韩昭昭[2] 王朝英[3] 朱广瑾[1] 解思深[3] 许海燕[2] 

机构地区：[1]中国医学科学院基础医学研究所,北京协和医学院基础医学院病理生理系,北京市100005 [2]中国医学科学院基础医学研究所,北京协和医学院基础医学院生物医学工程系,北京市100005 [3]中国科学院物理所,北京市100080

出　　处：《中国组织工程研究与临床康复》2008年第49期9691-9694,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(90606018)~~

摘　　要：应用电纺丝方法制备纤维直径为300～500nm的多壁碳纳米管/聚氨酯复合材料,以无纺膜材料作为细胞支架,选择在促进组织修复和再生中起重要作用的成纤维细胞株作为实验细胞。通过扫描电镜对多壁碳纳米管/聚氨酯无纺膜及聚氨酯无纺膜的微观形貌进行表征;通过细胞黏附实验、增殖实验以及细胞骨架发育观察,探讨无纺膜的微观纳米拓扑结构及多壁碳纳米管的复合对细胞的作用;并进一步采用双层细胞培养装置,分析多壁碳纳米管/聚氨酯无纺膜通过细胞通讯途径对在其他材料上生长的细胞生长行为的影响。实验结果表明,无纺膜中的纳米纤维网络结构和多壁碳纳米管成分不仅能够显著促进细胞的黏附和增殖,而且有利于细胞的迁移和聚集;另外,生长在多壁碳纳米管/聚氨酯无纺膜支架上的细胞可能通过旁分泌方式将某些生物大分子分泌到细胞外液中,经局部扩散作用于在其他材料上生长的细胞,促进其增殖。因此,多壁碳纳米管/聚氨酯纳米纤维无纺膜为细胞提供了接近天然细胞外基质的人造微环境,显示了该支架在引导组织修复和再生中的应用潜力。In this paper, the effect of nanofibrous structure and multiwalled carbon nanotubes （MWNTs） incorporation in the polyurethane （PU） on the fibroblasts growth behavior was studied. The nonwoven MWNT/PU scaffold with an average fiber diameter of 300 nm500 nm was fabricated by electrospinning technique. The nonwoven PU scaffold, MWNT/PU casting films and PU casting films were also prepared as controls. Cell viability assay, laser confocal microscopy and scanning electron microscopy were applied to evaluate cell adhesion, proliferation and cytoskeletal development on the scaffolds, respectively. Additionally, an obvious promotional influence of the cells cultivated in nonwoven MWNT/PU scaffold upon the proliferation of those growing in the other kind of substrate through cell-cell communication had been found. Experimental results demonstrated that the scaffold with nanofibrous structure and MWNTs incorporation exhibited highest enhancement not only to the cell adhesion and proliferation, but also to the cell migration and aggregation. Hence, the nanofibrous architecture and MWNTs incorporation provided favorite interactions to the ceils, which implied the application potentials of the composite nanofibrous scaffold for tissue repair and regeneration.

关 键 词：碳纳米管 聚氨酯复合材料 纳米纤维结构 细胞生长 

分 类 号：R318[医药卫生—生物医学工程]