天鹅记忆接骨器对实验性骨折愈合过程中局部胰岛素样生长因子Ⅰ含量的影响(英文)  

Influence of swan-like memory connector on the contents of local insulin-like growth factorⅠ during fracture union

作　　者：王仁[1] 禹晓东[1] 付青格[2] 许硕贵[2] 张春才[2] 

机构地区：[1]解放军第四五一医院骨科,陕西省西安市710054 [2]解放军第二军医大学长海医院骨科,上海市200433

出　　处：《中国组织工程研究与临床康复》2008年第52期10397-10400,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:天鹅记忆接骨器能对长骨干形成三维立体固定,保证骨折端稳定,并在骨折端间产生持续动态的压应力。胰岛素样生长因子Ⅰ对于骨生长代谢、细胞分化和有丝分裂都有调节作用,在骨折愈合过程中能促进细胞增生,刺激骨和软骨形成。目的:定量检测天鹅记忆接骨器与传统固定方式动力加压接骨板内固定下骨折局部胰岛素样生长因子Ⅰ的表达变化。设计、时间及地点:对照观察动物实验,于2007-01/05在解放军第二军医大学长海医院骨科实验室完成。材料:天鹅记忆接骨器为含镍50%～53%的镍钛合金板材,形状恢复温度为(33±2)℃,由兰州西脉生物医疗器械公司定制。方法:选用成年新西兰白兔30只,随机选择兔一侧肱骨,截骨后用天鹅记忆接骨器固定;对侧肱骨同法截骨后,在相应位置用动力加压接骨板固定。内固定后第2,3,4,6,8,12周分别处死5只动物,以骨折线两侧0.5cm范围内为取材区,标本按内固定物分为天鹅记忆接骨器组和动力加压接骨板组。主要观察指标:天鹅记忆接骨器、动力加压接骨板内固定后2,3,4,6,8,12周兔骨折局部胰岛素样生长因子Ⅰ含量。结果:天鹅记忆接骨器组骨折局部胰岛素样生长因子Ⅰ含量在内固定后2周逐渐增高,6周时达到顶峰,8～12周逐渐下降。动力加压接骨板组骨折局部胰岛素样生长因子Ⅰ含量在内固定后2周逐渐增高,8周时达到顶峰,12周下降。内固定后2,8,12周,两组骨折局部胰岛素样生长因子Ⅰ含量差异无显著性意义(P>0.05);内固定后3,4,6周,天鹅记忆接骨器组骨折局部胰岛素样生长因子Ⅰ含量明显高于动力加压接骨板组(P<0.05),4周时差异最大。结论:与传统固定方式动力加压接骨板相比,天鹅记忆接骨器独特的持续动态加压应力和三维立体固定能明显促进骨折局部胰岛素样生长因子Ⅰ的分泌,促进骨折愈合。BACKGROUND： Swan-like memory connector （SMC） can form a three-dimensional fixation on long bone shaft, ensure the stability of fracture ends, and produce a continuous dynamic compressive stress on the fracture ends. Insulin-like growth factor Ⅰ （IGF- Ⅰ ） regulates bone development and metabolism, cell differentiation, as well as mitosis. During the union process of fracture, IGF- Ⅰ is effective to promote cellular proliferation, stimulate bone formation and result in chondrogenesis. OBJECTIVE： To quantitatively determine the effect of SMC and conventional dynamic compression plate （DCP） on the concentration of local IGF- Ⅰ in the union duration of rabbit humerus fracture. DESIGN, TIME AND SETTING： A controlled observational animal study was performed in the laboratory, Department of Orthopaedics, Changhai Hospital, the Second Military Medical University of Chinese PLA from January to May in 2007. MATERIALS： SMC was Ni-Ti alloy panel, comprising 50%-53% Ni, was customized by Lanzhou Seemine Shape Memory Alloy Co., Ltd. The temperature of shape recovery was （33±2） ℃. METHODS： Thirty adult New Zealand rabbits were used in this study and were processed into osteotomy. The experiment sides of rabbit humerus were cut and fixed with SMC at random, serving as SMC group, and the other sides were cut at the same position and fixed with DCP, serving as DCP group. Each five animals were put to death at the time points of 2, 3, 4, 6, 8, and 12 weeks after internal fixation. Specimens were obtained from the areas that were 0.5 cm besides the fracture line. MAIN OUTCOME MEASURES： Following internal fixation, the contents of local IGF- I in the specimens of different time points （2, 3, 4, 6, 8, and 12 weeks） were determined in both SMC group and DCP group. RESULTS： In the SMC-treated group, the contents of IGF- 1 increased 2 weeks after internal fixation, reached the maximum at 6 weeks, and then decreased at 8-12 weeks. In the DCP-treated group, the contents of IGF-Ⅰ increased 2 wee

关 键 词：天鹅记忆接骨器 胰岛素样生长因子Ⅰ 骨折愈合 

分 类 号：R318[医药卫生—生物医学工程]