高血压糖尿病大鼠心脏肾上腺素受体的改变与心功能变化之间的关系  

Relationship between the Change of Cardiac Adrenoceptor and Cardiac Function in Hypertensive-diabetic Rats

作　　者：宫海滨[1] 张幼怡[2] 王雷[1] 王秀力[1] 王洁[1] 

机构地区：[1]徐州市心血管病研究所徐州市中心医院,江苏徐州221009 [2]北京大学第三医院血管医学研究所,北京100083

出　　处：《现代生物医学进展》2009年第2期223-225,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30572073);江苏省自然科学基金项目(DK2005428);江苏省卫生厅重大项目(K200402);江苏省医学重点人才资助项目(RC2007024)

摘　　要：目的:研究高血压糖尿病大鼠心脏肾上腺素受体(AR)的改变与心功能变化之间的关系。方法:采用左肾动脉缩窄和注射链脲佐菌素制高血压糖尿病大鼠模型,放射配体结合实验和离体左心房收缩功能实验等方法观察心脏AR(β-AR和/或α1-AR)及功能的改变。结果:与正常对照相比,高血压糖尿病大鼠心脏β-AR的最大结合容量(Bmax)增加35%(P<0.01),KD值不变;且心脏α1-AR的Bmax也显著增加(P<0.05)。高血压糖尿病大鼠左心房与对照相比,β-AR介导的最大收缩反应(Rmax)下降48%(P<0.01),pD2值不变;α1-AR介导的最大收缩反应也降低41%(P<0.05),pD2值不变。结论:高血压糖尿病大鼠心脏β-AR和/或α1-AR数量代偿性增加,但其介导的最大收缩反应降低,可能与受体后信号转导效应减弱有关。Objective： To study the relation of alteration of cardiac adrenoceptor （AR） with cardiac function in hypertensive-diabetic rats. Methods： The model of rats was induced by streptozotocin （STZ）, and the levels of cardiac AR （ β-AR and/or α1-AR） and cardiac fimction were detected by radioligand binding assays and functional determination of isolated left atria. Results： Compared with the control, the 13-AR density （Bmax） was increased by 35 % （p〈0.01） and the KD value was not changed in hypertensive-diabetic rats. Even the Bmax of α1-AR was increased significantly （p〈0.05）. The maximal positive inotropic response （Rmax） induced by isoproterenol in left atria of rat was decreased by 48 % （p〈0.01）, and α1-AR mediated Rmax was also decreased by 41%（P〈0.05）. Both ofpD2 value was not altered in isolated elective field driven left atria of hypertensive-diabetic rats. Conclusion： The results suggested that the Bmax of cardiac β-AR and/or α1-AR was increased in compensation, then AR mediated Rmax was decreased in diabetic rats. This may be associated with the reduction of post-receptor signal transduction.

关 键 词：高血压 糖尿病 肾上腺素受体 心脏 

分 类 号：R587.1[医药卫生—内分泌]