免疫纳米荧光微粒的制备和在中药研究中的应用  

The production of Immunologic Nano-Fluorescence beads and the application in traditional Chinese medicine

作　　者：杨兵[1] 李兴玉[1] 李静[1] 杨帆[1] 陈婧婧[1] 李春炎[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《现代生物医学进展》2009年第2期251-254,共4页Progress in Modern Biomedicine

基　　金：上海高校发展基金(05ZD21)

摘　　要：目的:制备免疫纳米荧光微球(Immunologic Nano-Fluorescence beads,INFB)并对其粒径和功能进行了研究。方法:应用纳米生物技术、免疫学技术和现代细胞生物学研究技术进行研究。选用K562细胞和Hela细胞为靶细胞,经INFB作用48h后,MTT法检测各组OD值。选用苏木、大血藤、秦巴西菇水提液和茶多糖诱导脐血单个核细胞,用INFB标记,FACS检测结果。结果:扫描电镜显示,荧光微球粒径平均为136.9nm。MTT结果显示,各实验组数据与对照组比较,无统计学意义(p>0.5),表明INFB对所选靶细胞无生物毒性作用。中药有效成分诱导人脐血单个核细胞后,CD34、CD33、CD14、CD119、CD19、CD4阳性细胞的数值,呈现剂量依赖关系,经统计学分析,药物浓度与检测的靶细胞数量呈现良好的线性关系(r=0.745376～0.986402)。结论:INFB可用于医学检验学、免疫学标记和中药活性成分的靶向作用研究等领域。Objective： To prepare immunologic nano-fluorescence beads （INFB） and research their size and function. Methods： Nano-biotechnology, immunology technology and modem cell biology research techniques were applied in the research. K562 cells and Hela cells were used as target cells. After they pretreated with INFB for 48 h, OD value were detected by MTT. HE, Sargentodoxa cunea- ta, Brazil agaricus mushroom water extract （ABWE） and tea polysaccharide were used in inducing cord blood mononuclear cells, and re- sults were detected by FACS. Results： Fluorescent microspheres testify showed the mean diameter of INFB was 136.9 nm. Compared with the control group, the experimental data was no significance. It showed that 1NFB had no biological toxicity on the selected target cells. The results of CD34^＋, CD33^＋, CD14^＋, CD119^＋, CD19^＋, CD4^＋ showed dose-dependent relationship, and the drug concentration showed a good linear relationship with the amount of detected target cells （r=0.745376-0.986402） by statistical analysis. Conclusion： INFB can be applied in the domain of medical tests, immunology technology and detecting the active component of the traditional Chinese medicine.

关 键 词：免疫纳米荧光微球(INFB) FACS 脐血单个核细胞 免疫细胞 

分 类 号：Q952.6[生物学—动物学]