黄芪总皂苷的纯化工艺  

Purification Process of Total Saponin from Radix Astragali

作　　者：赖先荣[1] 吴秦西[2] 向永臣[1] 

机构地区：[1]成都中医药大学,四川成都611137 [2]成都市第五人民医院,四川成都611130

出　　处：《现代生物医学进展》2009年第2期301-304,共4页Progress in Modern Biomedicine

基　　金：国家高技术研究发展计划(863计划)(2002AA2Z3204)

摘　　要：目的:优化黄芪总皂苷的纯化工艺。方法:以总皂苷含量为指标,采用均匀设计法对黄芪总皂苷的纯化工艺参数进行优化。结果:最佳纯化工艺条件为:药液浓度为1g原生药/ml,加入氢氧化钠至5%,加入水饱和正丁醇动态萃取2次,每次1.25倍量,加5%氢氧化钠溶液1倍量动态洗涤1次,加水1倍量动态洗涤2次,调节正丁醇溶液pH至中性。优化条件下的验证试验得到的黄芪总皂苷含量平均值为65.23%(n=3)。结论:建立的黄芪总皂苷的纯化工艺稳定、可行,适合放大生产。Objective： To investigate the optimum parameters to purify total saponin from Radix Astragali. Methods： the content of total saponin was set as the target.The uniform design method was applied to investigate the optimization process of purification. Results： The optimum condition of purifying the total saponin was obtained： the extract solution concentration of Radix Astragaliwas set at 1 g/ml; water saturation butanol was added to dynamic extract for two times with one and one-quartertimes of the volume each time; five percent of sodium hydroxide solution was added and given dynamic washing for one time; the water was used for dynamic washing for two times;then the butanol solution was regulated to neutral pH.The average content of total saponin was 65.23 % at the optimum purificaton condition in verification experimentations（n=3）. Conclusions： The optimum parameters to purify the total saponin from Radix Astragali showed good stabilization and feasibility,and can be available for industrial production at large scale.

关 键 词：黄芪 总皂苷 纯化工艺 均匀设计 

分 类 号：R284.2[医药卫生—中药学]