内生黄曲霉对丹参悬浮细胞氧化酶活性的影响  

Effect of Endophytic Aspergillus flavus on Activity of Oxidases in Cell Suspension Culture of Salvia miltiorrhiza Bunge

作　　者：杨华[1] 郭纯[2] 刘高[1] 饶力群[1] 彭国平[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]湖南中医药大学药学院,湖南长沙410007

出　　处：《现代生物医学进展》2009年第2期312-314,共3页Progress in Modern Biomedicine

基　　金：湖南农业大学校青年基金资助项目(06QN14)

摘　　要：目的:研究内生黄曲霉对丹参悬浮细胞次生代谢相关氧化酶活性影响,探讨丹参组织培养和次生代谢产物产生的有效诱导途径和诱导机理。方法:将丹参内生黄曲霉以活菌、发酵菌液及制作成诱导子方式分别添加到丹参悬浮细胞中,测定丹参悬浮细胞过氧化物酶、过氧化氢酶及多酚氧化酶活性。结果:不同处理方式,内生黄曲霉对丹参悬浮细胞氧化酶表达均有明显的诱导作用,其中将黄曲霉制作成诱导子对三种酶活诱导作用最明显。结论:内生黄曲霉诱导子能有效提高丹参悬浮细胞氧化酶活性。Objective： To study the effects of endophytic aspergillus flavus on activity of oxidases in cell suspension culture of Salvia miltiorrhiza Bunge. Methods： Live aspergillus flavus, sinensi and elicitor were used to treat the suspension cells respectively, and the activities ofperoxidase （POD） ,calatase（CAT） and polyphenoloxidase（PPO） in the suspension cells were determined. Results： The activities of three oxidases in the suspension cells of Salvia miltiorrhiza Bunge were induced significantly by endophytic aspergillus flavus with different treatments.Elicitor had most significant effect. Conclusions： Elicitor from endophytic aspergillus flavus could enhance the activities of some oxidases in cell suspension culture of Salvia miltiorrhiza Bunge.

关 键 词：丹参 内生菌 氧化酶 

分 类 号：R285.5[医药卫生—中药学]