低剂量γ射线辐照离体全血对血清抗氧化酶活性的影响  

Effect of low dose gamma ray irradiated blood on the enzyme activities of serum antioxidases

作　　者：夏爱军[1] 张献清[1] 穆士杰[1] 易静[1] 吴原茹[1] 

机构地区：[1]第四军医大学西京医院输血科,西安710032

出　　处：《临床血液学杂志（输血与检验）》2009年第1期68-69,共2页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨低剂量γ射线辐照人全血对血清抗氧化酶活性的影响。方法:20份人全血,以剂量为1Gy的γ射线进行辐照,吸收剂量率为17Gy/min,分别于照射前及照射后1、2h取样检测血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)、过氧化氢酶(CAT)活力。结果:离体全血在剂量为1Gyγ射线照射后1h,可见血清中SOD、GR、GSH-Px及CAT活性升高,且均与照射前比较差异有统计学意义(P<0.01);经γ射线照射2h后,除SOD活性与照射后1h差异无统计学意义,GR、GSH-Px及CAT活性进一步升高,与照射后1h比较差异有统计学意义(P<0.01)。结论:离体全血经1Gyγ射线辐照后,血清中主要抗氧化酶活性增高,因此增强抗自由基防御反应。Objective：To investigate the effects of Gamma ray irradiated blood on the enzyme activities of serum antioxidases. Method：Twenty units of whole blood were irradiated by γ- ray at a dose of 1Gy. The absorption dose rate was 17 Gy/min. The enzyme activities of serum superoxide dismutase（SOD）, glutathione peroxidase （GSH-Px） ,glutathione reductase（GR）,catalase（CAT）were detected before irradiation and 1 h, 2 h after irradiation respectively. Result：One hour after irradiation, the enzyme activities of serum SOD, GSH-Px, GR and CAT were higher than those of pre-radiation （P〈0.01）. Two hours after radiation, enzyme activities of all antioxidases were higher than those of one hour after radiation, except SOD （P〈0.01）. Conclusion： Gamma ray irradiation at low dose can obviously enhance the enzyme activities of serum antioxidases, therefore enhance defence reaction of anti free radical.

关 键 词：Γ射线 照射 抗氧化 

分 类 号：R457.1[医药卫生—治疗学] R815.2[医药卫生—临床医学]