雪旺细胞体外培养改良新方法的实验研究  

The empirical study on the modified method of Schwann cell culture in vitro

作　　者：洪楷[1] 温凤金[1] 翁惠兰[2] 吴利标[2] 

机构地区：[1]广东汕头大学医学院附属第二医院,515041 [2]广东汕头大学医学院附属第一医院,515041

出　　处：《国际检验医学杂志》2009年第1期1-2,6,共3页International Journal of Laboratory Medicine

基　　金：广东省汕头市科技计划项目(2005116021)

摘　　要：目的研究一种简便、快速的能获取高纯度雪旺细胞(Schwann cells,SCs)的体外培养新方法。方法采用Ⅳ型胶原酶和胰蛋白酶消化出生3 d的SD鼠臂层神经和坐骨神经组织块后再将组织块贴壁培养的方法。结果用新方法培养的SCs"爬山"速度较直接组织贴壁培养方法快,且纯度高达98%,明显高于直接组织贴壁培养方法和酶组织块消化培养方法。结论经过改良的培养新方法简便可行,能为修复周围神经缺损的临床研究提供大量高纯度的SCs。Objective To search for a convenient and effective in vitro cell culture method to acquire highly purified Schwann cells. Methods The sciatic and brachial plexus perve of three-day-old SD mice was treated with collagenase type Ⅳ and trypsin to remove the desmocytes on the Schwann cells, then explanted in the culture disk. Results Schwann cells grew more quickly from the treated nerve than the nerve without treating; the purity of Schwann cell population was up to 98%, significantly higher than the tissue adherent culture and tissue enzymatic digestion culture methods. Conclusion The modified method is convenient and feasible, may acquire a great quantity of highly purified Schwann cells for clinical research on repair of peripheral nerve defect.

关 键 词：雪旺细胞 培养技术 方法 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]