乙型肝炎病毒表面抗体定性测定S/CO的临床意义  

The clinical significance of S/CO qualitative determination of hepatitis B surface antibody

作　　者：石鹏辉[1] 李永勤[1] 耿坤静 

机构地区：[1]河北省保定市传染病医院检验科,071000

出　　处：《国际检验医学杂志》2009年第1期14-15,19,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨乙肝病毒表面抗体定性测定S/CO(标本吸光度/临界值)值与定量测定值的相关性。方法时间分辨荧光免疫分析法(TRF)及酶联免疫吸附试验(ELISA)检测不同浓度的血清,绘制标准曲线,建立方程。结果S/CO值与定量测定值呈曲线正相关,转换方程式为Y=8.911 8×e^(0.177 4X)。S/CO计算值与实测值组内相关系数为0.934。结论乙肝病毒表面抗体含量可通过定性S/CO值推算,适用于基层未开展乙肝标志物定量检测的实验室应用。Objective To study the relativity of S/CO qualitative determination of hepatitis B surface antibody and quantitative assay. Methods The different concentrations of sera were determined with time-resolved fluorescence immunoassay and enzyme-linked immunosorbent assay. The standard curve was drawn and the equation was established. Results In the curve, S/CO level was positively correlative to the quantitative detection value, and the conversion equation was Y= 8.911 8 × e^0.1774X The intraclass correlation coefficient of S/CO calculating level and quantitative value was 0. 934. Conclusion The content of hepatitis B surface antibody can be estimated by S/CO value. It can be used in basic-level laboratories which don＇t undertake the quantitative determination of HBV markers.

关 键 词：肝炎抗体 乙型 酶联免疫吸附测定 标本 定性研究 

分 类 号：R446.6[医药卫生—诊断学] R512.62[医药卫生—临床医学]