可卡因对性成熟期大鼠睾丸谷胱甘肽过氧化物酶基因表达的影响  

The effect of cocaine on the gene expression of GSH-PX in testis of sexual maturity rats

作　　者：贾晓俤[1] 宋天保[2] 史小莲[3] 

机构地区：[1]西安交通大学医学院法医系,西安710061 [2]西安交通大学医学院生殖医学中心 [3]西安交通大学医学院药理系

出　　处：《中国药物滥用防治杂志》2009年第1期18-21,共4页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：陕西省自然科学基金资助(项目编号:2006C231)

摘　　要：目的:研究可卡因对大鼠睾丸谷胱甘肽过氧化物酶(glutathione peroxidase GSH-PX)基因表达的影响。方法:性成熟期健康SD雄性大鼠皮下注射可卡因制造吸毒动物模型。化学比色法检测睾丸氧化应激指标;半定量RT-PCR方法检测睾丸组织的GSH-PX mRNA表达。结果:可卡因处理第7天,实验组GSH-PX活性、MDA含量与对照组比较无显著性差异(P>0.05);而GSH-PX mRNA水平显著高于对照组(P<0.05)。可卡因处理第14天,实验组GSH-PX活性显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05),而GSH-PX mRNA水平显著高于对照组(P<0.05)。可卡因处理第21天,实验组GSH-PX活性及 mRNA表达水平显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05)。可卡因处理第28天,实验组GSH-PX活性及mRNA表达水平显著低于对照组(P<0.05),MDA含量显著高于对照组(P<0.05)。结论:可卡因对大鼠睾丸的损伤作用可能与其抑制GSH-PXmRNA基因表达有关。Objective： To study the effects of cocaine on the gene expression of glutathione peroxidase （GSH-PX） in testis of sexual maturity rats. Methods： Forty sexual maturity rats were randomly divided into 2 groups.One group served as control. The other group as cocaine exposure group was given cocaine hydrochloride （15 mg/kg body weight ） subcutaneously daily, cocaine exposure group were harvested at 1,2,3 and 4 weeks after treatment. GSH-PX mRNA expression was investigated by semi-quantitative RT-PCR. The level of GSH-PX and MDA in testis was evaluated by using spectrophotometric determination. Results： At first week after treatment,as compared with the control group,the mRNA expression level of GSH-PX in cocaine exposure group increased significantly （P〈0.05）,while the levels of GSH-PX and MDA mildly increased,but no statistically significant difference was observed.At second week after treatment, as compared with the control group,the mRNA expression level of GSH-PX and the level of MDA in cocaine exposure group increased significantly（P〈0.05）, while the level of GSH-PX decreased significantly（P〈0.05）.At third week after treatment,as compared with the control group, the mRNA expression level of GSH-PX and the level of GSH-PX in cocaine exposure group decreased significantly（P〈0.05）;while the level of MDA increased significantly（P〈0.05）. At forth week after treatment,as compared with the control group,the mRNA expression level of GSH-PX and the level of GSH-PX in cocaine exposure group decreased significantly（P〈0.05）;while the level of MDA increased significantly（P〈0.05）.

关 键 词：可卡因 基因表达 谷胱甘肽过氧化物酶 大鼠 

分 类 号：R96[医药卫生—药理学]