梨叶片中苹果褪绿叶斑病毒的引物原位标记检测  

Detection of Apple chlorotic leaf spot virus in Pear by Primed in situ Labeling

作　　者：赵英[1] 牛建新[1] 

机构地区：[1]石河子大学农学院,新疆石河子832003

出　　处：《园艺学报》2009年第1期15-20,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30360066);国家科技攻关计划引导项目(2003BA546C);兵团科委项目(NKB02SDXNK01SW)

摘　　要：选用带苹果褪绿叶斑病毒的香梨叶片,制备成适合进行原位RT-PCR的石蜡切片。设计特异引物,利用引物原位标记(Primedin situlabeling,PRINS)技术对切片组织中的病毒进行了检测研究,结果表明,PRINS-FITC染色法和PRINS-Rhodamine染色法均能获得良好的检测效果,有病毒部位分别显示绿色荧光和红色荧光,而且荧光出现的位置与原位RT-PCR检测的结果一致。由此证明引物原位标记技术可用于果树病毒的原位检测。The slices of pear leaf samples infected by Apple chlorotic leaf spot virus were prepared for in situ RT-PCR and were detected by primed in situ labeling technique using special primer. It was showed that both staining methods of PRINS-FITC and PRINS-Rhodamine can get good detection results in which the parts have viruses showed green and red fluorescence light, respectively, and the sites of showing fluorescence showed the same sites as the sites detected by RT-PCR method. Therefore, primed in situ labeling technique can be perfectly used for virus in situ detection of fruit trees, and it is also a rapid, simple and reliable in situ detection method.

关 键 词：梨 引物原位标记 苹果褪绿叶斑病毒 病毒检测方法 染色 

分 类 号：S661.2[农业科学—果树学]