附加甘蓝3号染色体的大白菜单体异附加系的获得与研究  

Obtaining and Studies of Chinese Cabbage Monosomic Alien Addition Line with Chromosome 3 of Cabbage

作　　者：顾爱侠[1] 郑宝智 王彦华[1] 轩淑欣[1] 罗双霞[1] 申书兴[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]邢台市农业科学院,河北邢台054000

出　　处：《园艺学报》2009年第1期39-44,共6页Acta Horticulturae Sinica

基　　金：河北省自然科学基金项目(C2005000222);河北农业大学将帅计划项目

摘　　要：以大白菜—结球甘蓝异源三倍体杂交种(AAC)与二倍体大白菜(AA)的回交一代为材料,利用根尖体细胞核型分析法鉴定出大白菜—结球甘蓝3号单体异附加系;利用甘蓝连锁群特异SSR标记鉴定表明,该单体异附加系中的外源染色体与甘蓝的03连锁群相对应;该单体异附加系植株矮小,花枝细弱倒伏,花粉整齐度、花粉生活力和结籽率均较低,花粉母细胞减数分裂过程中存在染色体不均衡分离及染色体落后等现象。The BC1 progenies derived from allotriploid hybrid （AAC） and diploid Chinese cabbage （AA） were used materials. The Chinese cabbage-cabbage monosomie alien addition line introducing chro- mosome 3 of cabbage was identified by karyotype analysis of root tip. The results from specific SSR markers for cabbage linkage groups compared with Chinese cabbage showed that the introduced cabbage chromosome in Chinese cabbage -cabbage monosomic alien addition line was grouped into linkage group 03. During repro- ductive growth, the monosomie alien addition line is short, the flowering branch is gracility and lodging, the pollen regularity, the pollen activity and seed set ratio are low. And there are a series of abnormal phenomena in meiosis of PMCs, such as chromosome unbalanced separation, backward chromosomes, and so on.

关 键 词：大白菜 结球甘蓝 单体异附加系 核型分析 SSR 

分 类 号：S634.1[农业科学—蔬菜学]