蛭形巨吻棘头虫的LDH同工酶  

Study on the LDH Isoenzyme Patterns of Macracanthorhynchus hirudinaceus

作　　者：廖党金[1] 石雁羽 王雄清[3] 许平 

机构地区：[1]四川省畜牧兽医研究所 [2]四川省职工科技大学 [3]乐山师范专科学校 [4]重庆师范学院

出　　处：《中国兽医学报》1998年第2期159-162,共4页Chinese Journal of Veterinary Science

基　　金：四川省科委资助

摘　　要：用聚丙烯酰胺凝胶电泳对蛭形巨吻棘头虫（Ｍａｃｒａｃａｎｔｈｏｒｈｙｎｃｈｕｓｈｉｒｕｄｉｎａｃｅｕｓ）雄虫、雌虫体液和宿主（猪）血清的ＬＤＨ同工酶的酶谱、相对迁移率和相对活性等进行比较表明，雄虫有３条酶带和雌虫有２条酶带显示出很强的特异性；不同浓度的抑制剂（硝硫氰醚）对雄虫和雌虫特有的ＬＤＨ同工酶酶带活性具有不同程度的抑制作用，尤其是雌虫第２带对抑制剂最敏感。蛭形巨吻棘头虫体液具有特异的且不同于宿主（猪）血清ＬＤＨ１～５的ＬＤＨ同工酶酶带，特有的ＬＤＨ同工酶可能由４条不同的区带组成。The LDH isoenzyme patterns of Macracanthorhynchus hirudinaceus male and female body liquids and the host (pig) serum were disconnected by the polyacrylamide gel disc electrophoresis. The comparsion of the pattern, relative migration rate and relative activities of their isoenzyme band showed that the three LDH isoenzyme bands of the male and the two LDH isoenzyme bands of the female were rather peculiar. On the other hand, the activities of LDH isoenzyme of the three materials were restrained by the different densities of the nitroscanate (inhibitor). The special LDH isoenzyme bands of the worm were affected by the inhibitor with the second LDH isoenzyme band of the female being the most sensitive to it indicating that the special LDH isoenzyme bands seem to be different from the LDH1 5 of the host serum. These showed that the special LDH isoenzyme band patterns in M. hirudinaceus body liquid may be of four types (four LDH isoenzyme bands).

关 键 词：蛭形巨吻棘头虫 同工酶 抑制试验 硝硫氰醚 

分 类 号：S852.732[农业科学—基础兽医学]