湖羊产羔性状的微卫星标记与可能生产力的关联性分析  

Relationship between Microsatellite Markers and Producing Ability on Litter Trait of Hu Sheep

作　　者：孙伟[1] 常洪[1] 金银[1] 王鹏[1] 钱建共[2] 吴文忠[2] 陈玲[2] 王伟 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]苏州市畜牧兽医站,苏州215128 [3]苏州市吴中区动物卫生监督所,苏州215128

出　　处：《畜牧兽医学报》2009年第1期7-14,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然基金国际合作项目(30410103150);中国博士后科学基金(20080430470);江苏省自然科学基金(BK2007556);国家科技基础条件平台项目(2005DKA21101);国家高技术研究与发展计划(863)(2006AA10Z198);国家“十一五”科技支撑(2006BAD13B08);“十一五”国家科技支撑计划重点项目(2008BADB2B04);江苏省教育厅基金项目(NK051039);江苏省政府留学奖学金;江苏省高校“青蓝工程”优秀青年骨干教师培养基金;扬州大学“新世纪人才工程”优秀骨干教师培养基金

摘　　要：选择位于绵羊第6号染色体上与多胎基因(FecB)紧密连锁的微卫星OarAE101、BM1329、BM143和Oar-HH55标记以及位于第4号染色体上的OarHH35标记对湖羊的产羔性状进行研究,结果显示:湖羊的5个微卫星座位都达到了高度多态水平(PIC>0.5),5个微卫星座位可以用于湖羊产羔性状的遗传多样性的评估。方差分析结果表明:5个基因座中OarAE101、BM143各基因型产羔数生产力之间存在极显著差异(P<0.01),OarHH35各基因型产羔数可能生产力之间存在显著差异(0.01<P<0.05),BM1329、OarHH55各基因型产羔数可能生产力之间差异不显著(P>0.05)。在OarAE101标记中107/113的产羔数最小二乘平均值为最大,与基因型103/113、107/107、101/111、113/113、107/111和103/103之间产羔数可能生产力最小二乘平均值差异不显著(P>0.05),但与101/101和103/107之间产羔数最小二乘平均值差异显著(0.01<P<0.05),与115/115和111/111之间产羔数可能生产力最小二乘平均值差异极显著(P<0.01)。在OarHH35标记中139/139的产羔数可能生产力最小二乘平均值为最大,与基因型139/141、139/127、137/125之间产羔数可能生产力最小二乘平均值差异不显著(P>0.05),但与127/127、125/125和139/125之间产羔数可能生产力最小二乘平均值差异极显著(P<0.01)。在BM143标记中118/118的产羔数可能生产力最小二乘平均值为最大,与基因型118/106、118/110、106/104和112/114之间产羔数可能生产力最小二乘平均值差异不显著(P>0.05),但与118/116和102/106之间产羔数可能生产力最小二乘平均值差异显著(0.01<P<0.05),与102/104、112/102和116/112之间产羔数可能生产力最小二乘平均值差异极显著(P<0.01)。研究将在今后湖羊品种的标记辅助选择和分子育种中具有重要的应用价值。Four microsatellite loci （OarAE101, BM1329, BM143, OarHH55） linked to Fec^B gene mapped to chromosome 6 and one microsatellite locus （OarHH35） on chromosome 4 were selected to study the litter trait in Hu sheep. Results showed that all 5 microsatellite loci reached high polymorphism （PIC〉0. 5）, and the five microsatellite loci can be used for genetic diversity evaluation in Hu sheep breed. The result of variance analysis showed that there were extremely significant difference among the different genotypes for the Producing ability of litter size in OarAE101 locus and BM143 locus（P〈0.01）, there were significant difference among the different genotypes for the Producing ability of litter size in OarHH35 locus（0.01〈P〈0.05）,and there were no significant difference among the different genotypes for the Producing ability of litter size in BM1329 and OarHH55 loci（P〉0.05）. Least squares mean for the Producing ability of litter size for OarAE101 107/113 was the most, and it is not significantly higher than those for OarAE101 103/113,107/107,101/111,113/113,107/111 and 103/103 （P〉0.05）, however it is significantly higher than those for OarAE101 101/101 and 103/107（0.01〈P〈0.05）, and it is extremely significant higher than those for OarAE101 115/115 and 111/111 （P〈0. 01）in Hu sheep. Least squares mean for the Producing ability of litter size for OarHH35 139/139 was the most, however it is not significantly higher than those for OarHH35 139/141,139/127 and 137/ 125 （P〉0.05）, and it is extremely significant higher than those for OarHH35 139/125,127/127, 125/125（P〈0.01）in Hu sheep. Least squares mean for the Producing ability of litter size for BM143 118/118 was the most, however it is not significantly higher than those for BM143 118/ 106,118/110,106/104 and 112/114 （P〉0.05）, it is significantly higher than those for BM143 118/116 and 102/106 （0. 01〈P〈0. 05） , and it is extremely significant higher than those for BM143 102/104,112/102 and116/112（

关 键 词：湖羊 微卫星标记 产羔性状 母羊可能生产力 

分 类 号：S826.2[农业科学—畜牧学]