传染性支气管炎病毒分离株的生物学特性鉴定及其遗传变异分析  

Biological Characteristics and Genetic Diversities Analysis of Infectious Bronchitis Virus

作　　者：吴培培[1] 唐应华[1] 刘文博[1] 陈义平[2] 张小荣[1] 吴艳涛[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009 [2]中国动物卫生与流行病学中心,青岛266032

出　　处：《畜牧兽医学报》2009年第1期89-92,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划项目(2006BAD06A11)

摘　　要：通过鸡胚矮小试验、鸡胚气管环组织培养试验、干扰新城疫病毒复制试验和动物回归试验，将2006-2007年在江苏省分离的7株鸡传染性支气管炎病毒（IBV）鉴定为嗜肾型毒株。采用RT-PCR扩增IBV分离株的S1基因，并进行克隆和序列分析。结果显示7个IBV分离株S1基因的核苷酸同源性为94．6％-99．4％，处于同一个群，分别属于3个亚群。这些毒株与大多数国内近年分离株的同源性较高，而与Massachusetts、T、4／91和793B血清型毒株（包括H120和H52疫苗毒株）的同源性较低。IBV流行毒株和疫苗毒株的差异是造成免疫鸡群发生传染性支气管炎的重要原因。Seven infectious bronchitis viruses（IBV） isolated from chicken in Jiangsu province between 2006 and 2007 were identified as nephropathogenic strains by dwarfing embryonated chicken eggs, chicken embryonated tracheal organ culture （TOC） assay, inhibition with NDV test and chicken recurrent infection. S1 gene of them were amplified by reverse transcription-polymerase chain reaction （RT-PCR）, subsequently, the amplified fragments were cloned into TA vector and sequenced. Analysis of the phylogenetic tree based on S1 gene showed that Jiangsu IBV isolates were clustered into one group, and attributed to three subgroups. Homology of S1 nucleotide sequence among seven viruses varied from 94.6% （between CK/CH/JS/06I and CK/CH/JS/06Ⅲ ） up to 99.4% （between CK/CH/JS/07Ⅲ and CK/CH/JS/07Ⅳ）. The viruses in this study shared high similarity with the viruses isolates in China recent years, but shared low similarity with vaccine strains （H120 and H52） and other serotype index strains （T, Mass, 793B, 4/91）. The genetic distance differences between IBV epidemic and vaccine strains lead to outbreak of the IB and circulate in immunized chicken.

关 键 词：传染性支气管炎病毒 生物学特性 S1基因 RT—PCR 

分 类 号：S852.696[农业科学—基础兽医学]