猪链球菌耐药性产生对其毒力的影响  

The Virulence Change of Streptococcus suis Resistant to Antimicrobials

作　　者：王丽平[1] 陆承平[1] 

机构地区：[1]南京农业大学动物医学院,南京210095

出　　处：《畜牧兽医学报》2009年第1期103-108,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家973项目子专题(G1999011906)

摘　　要：为探讨猪链球菌2型菌株耐药性产生对其毒力的影响,作者采用次抑菌浓度的青霉素和红霉素诱导猪链球菌2型临床分离敏感株HA9801成为青霉素、红霉素耐药株,并对亲本株与诱导耐药株的半数致死量(LD50)、溶血环、毒力因子溶菌酶释放蛋白(MRP)、胞外蛋白因子(EF)和荚膜多糖(CPS)及对小鼠的组织损伤进行比较。结果显示:次抑菌浓度的红霉素和青霉素可以诱导耐药性的产生,但耐药株与亲本株比较,溶血性、LD50、MRP、CPS、EF及对组织的损伤程度均与亲本株无显著差异(P<0.05)。结果表明:猪链球菌2型HA9801株在获得耐药性的同时,仍能保持与亲本株相似的毒力,其毒力不会因耐药性的获得而降低。The study was designed to investigate the relationship between virulence and antimicrobial resistance of Streptococcus suis. The difference of the growth rate, LD50, haemolytic ring, histological damage and PCR of virulent factors such as MRP, CPS and EF between parental and resistant strains were determinated. The results showed that Streptococcus suis HA9801 strain can be induced into resistant strain when exposing to penicillin and erythromycin at sub-MIC concentration. Resistant strains is still virulent, as demonstrated experimently in mice by quantitative evidence of tissue damage,LD50 and in vitro by growth rate, haemolytic ring and virulent factors（ MRP,CPS and EF） by PCR method between parental strain and induced-resistant strains. The results draw attention to the risk that the pathogenicity of the strains can not be reduced in Streptococcus suis type 2, the HA9801 resistant strain, to some extent, the strain can still cause infection.

关 键 词：猪链球菌2型 毒力 耐药 LD50 溶血性 毒力因子 组织损伤 

分 类 号：S852.611[农业科学—基础兽医学]