不同细胞因子对山羊卵母细胞体外成熟与早期胚胎发育的影响  

Effect of Cytokine on in vitro Maturation of Oocytes and Early Embryos Development in Goat

作　　者：赵振华[1,2] 周敏敏[1] 钱红娟[1] 王杏龙[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]中国农业科学院家禽研究所,扬州225003

出　　处：《畜牧兽医学报》2009年第1期133-137,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：研究在培养液中添加不同浓度的GDNF、LIF、SCF及其不同组合对山羊卵母细胞体外成熟和早期胚胎发育的影响。结果表明:SCF、LIF、GDNF对山羊卵母细胞体外成熟有显著影响(62.0%、75.5%、63.7%vs 53.0%,P<0.05),不同浓度间无显著影响(P>0.05),添加LIF、GDNF对卵裂率有显著影响(48.1%、43.3%vs 26.2%,P<0.05),SCF对卵裂率无显著影响(37.6%vs 26.2%P>0.05),不同细胞因子都能显著提高囊胚率(21.6%、27.3%、26.2%vs6.3%,P<0.05)。10 I U.mL-1的LIF、15 I U.mL-1的GDNF和15 I U.mL-1的SCF对山羊胚胎体外生产最好;GDNF+LIF+SCF组和GDNF+LIF组卵母细胞成熟率显著高于LIF+SCF,GDNF+SCF,GDNF和LIF组(83.3%、80.3%vs 72.8%、65.4%、63.3%、77.9%,P<0.05),GDNF+SCF和LIF+SCF添加组间对卵母细胞体外成熟无差异(72.8%vs 65.4%,P>0.05),早期胚胎发育上GDNF+LIF+SCF组优于GDNF+LIF、LIF+SCF和GDNF+SCF组,且均能显著提高卵裂率(50.2%、48.9%、47.9%、43.7%vs 21.4%,P<0.05)和囊胚率(31.3%、27.6%、25.5%、24.3%vs 13.6%,P<0.05)。可见GDNF、LIF和SCF能提高卵母细胞的成熟率,早期胚胎的卵裂率和囊胚率,LIF和GDNF对卵母细胞体外成熟有协同作用。Effects of GDNF, LIF and SCF separation or combination on in vitro maturation of goat oocytes and early embryos development were studied. The results showed that there was significant difference between control group and experiment groups in maturation rate（62.0%, 75.5% ,63.7% vs 53.0% ,P〈0.05）, but no significant difference between different solutions（P〉0.05）. LIF and GDNF groups were significantly different from control group（48.1%, 43.3% vs 26.2%,P〈0.05）, no difference between control group and SCF group. Experiment groups significantly improved the blastocyst formation rate（21.6%, 27.3%,26.2% vs6.3% ,P〈0.05）. The 10 IU· mL^-1 LIF, 15 IU·mL^-1 GDNFand 15 IU· mL^-1 SCF were the best solutions for goat IVP. Maturation rate of goat oocytes was improved significantly in the groups with GDN＋ LIF＋SCF and GDNF＋ LIF than that in the groups with GDNF＋SCF, LIF＋ SCF, GDNF, LIF （83.3%,80.3% vs 72.8%,65.4%,63.3%,77.9%,P〈0.05）. GDN＋ LIF＋SCF and GDNF＋ LIF groups were better than LIF ＋ SCF and GDNF＋SCF groups at cleavage rate （50.2%,48.9%,47.9%,43.7% vs 21.4%,P〈0.0） and blastocyst rate（31.3%,27.6%,25.5%,24.3% vs 13.6% ,P〈0.05）. The results obtained in this study indicated that GDNF, LIF and SCF significantly improved oocytes maturation and the blastocyst formation rate in IVP of goat. LIF and GDNF have coeffects on in vitro maturation of goat oocytes.

关 键 词：胚胎 体外受精 体外生产 山羊 

分 类 号：S823.3[农业科学—畜牧学]