奶牛子宫内膜炎大肠杆菌耐药性及其与整合子关系的研究  

Research of Drug-resistance of Escherichia coli Isolated from Bovine with Endometritis and the Relationship between Antibiotic Resistance and Integron

作　　者：赵红霞[1] 李培锋[1] 吴聪明[2] 樊宏亮 沈建忠[2] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018 [2]中国农业大学动物医学院,北京100193 [3]内蒙古奈伦天然乳品有限公司,呼和浩特010070

出　　处：《畜牧兽医学报》2009年第1期142-144,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“十一五”科技支撑计划项目(2006BAK02A03)

摘　　要：为阐明奶牛子宫内膜炎大肠杆菌的耐药性及其与细菌携带整合子之间的关系,采用微量稀释法测定药物对各菌株的最小抑菌浓度,以确定菌株的耐药性;运用PCR方法检测细菌携带I类整合子的情况。结果:所有菌株对头孢噻呋、呋喃唑酮以及喹诺酮类药物的耐药率较低,均小于35%,而对磺胺类和抗菌增效剂的耐药率较高,均在97%以上;57株大肠杆菌中有21株携带I类整合子,阳性率为36.8%,且I类整合子阳性菌株均为多重耐药菌株(耐8种以上抗菌药物);整合子阳性菌株对链霉素、阿莫西林、氨苄西林、头孢噻吩、四环素、土霉素的耐药率均显著高于整合子阴性菌株。结果表明奶牛子宫内膜炎致病性大肠杆菌的耐药性和多重耐药性与整合子携带有着密切关系。The drug-resistance of Escherichia coli isolates from dairy cows with endometritis in Huhhot and the relationship between antibiotic resistance of these strains and integron which they carrying were researched. The drug-resistance of the isolates was defined by detecting MIC of every strain; The integrons in these E. coli isolates were investigated by PCR technique. 56 isolates of 10 serogroups were pathogenic strains. 21 of 57 strains were identified as positive for class Ⅰ integron, account for 36.8%. The integron-bearing isolates were all multi-resistance strains, and showed tolerance to more than eight kinds of antibacterial drugs. The results showed that the resistance rates of strains positive for integron Ⅰ were remarkably higher than that of isolates negative for integron Ⅰ. The resistance rates of the isolates to ceftiofur, furazolidone and quinolones were all lower than 35%; They were all resistant to sulfonamides and trimethoprim, the resistant rates were above 97 %. The results revealed that the drug-resistance and multi-resistance of pathogenic E. coli from bovines with endometritis in Huhhot were strongly correlated with the integron Ⅰ.

关 键 词：奶牛子宫内膜炎 大肠杆菌 致病性 耐药性 整合子 

分 类 号：S852.612[农业科学—基础兽医学]