UV法测甘草黄酮微丸含量  

UV spectrophotometry determination of content of glycyrrhiza total flavones pellets

作　　者：陈丽华[1] 徐德生[2] 冯怡[3] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [2]上海中医药大学曙光医院,上海200021 [3]上海中医药大学,上海201203

出　　处：《药物分析杂志》2009年第1期128-129,共2页Chinese Journal of Pharmaceutical Analysis

基　　金：上海市重点学科建设项目资助(项目编号:T0301);上海市科委项目资助(项目编号:04DZ19841);高等学校博士学科点专项科研基金资助项目(项目编号:20060268009)

摘　　要：目的:建立甘草黄酮微丸的含量测定方法。方法:采用紫外分光光度法,以甘草苷为指标性成分,在335nm波长处进行含量测定。结果:测定微丸中甘草黄酮含量以甘草苷计为36.2%,甘草苷在4.07～20.35μg范围内线性关系良好(r=0.9997),平均回收率(n=9)为98.6%。结论:本测定方法简便、快速,准确性和重复性好。Objective ：To establish a method for determining the quantitative of glycyrrhiza total flavones pellets by UV spectrophotometry. Method：Liquiritin was used as the reference component for UV spectrophotometry, determined at 335 nm. Results：The quantitative of total flavones from pellets calculated with reference to liquiritin was 36. 2% ;The linear range of liquiritin was 4. 07 -20. 35 g（r =0. 9997） ;The average recovery（n =9） was 98. 6%. Conclusion：The method is simple, accurate and reliable for determining the quantitation of glycyrrbiza total flavones.

关 键 词：甘草黄酮微丸 甘草苷 紫外分光光度法 

分 类 号：R917[医药卫生—药物分析学]