舒血宁注射液中总银杏酸的HPLC法限量检查  

Limit test of total ginkgolic acid in Shuxuening injection by HPLC method

作　　者：杜小伟[1] 王京辉[1] 王萌萌[1] 张小茜[1] 荆芳 房秀霞 王海涛 王连喜 

机构地区：[1]北京市药品检验所,北京100035 [2]北京双鹤高科天然药物有限责任公司,北京102100

出　　处：《药物分析杂志》2009年第1期170-172,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立舒血宁注射液中总银杏酸的限量检查方法,为舒血宁注射液质量标准相关检测项目提供检测方法和试验依据。方法:样品以石油醚(60～90℃)振摇提取,回收溶剂,甲醇溶解,制备供试品溶液;色谱分析条件:色谱柱为InertsilODS-3C18柱(4.6mm×150mm,5μm),流动相为乙腈-0.1%磷酸溶液(85:10),流速1mL.min-1,检测波长300nm。结果:白果新酸进样量在4.4～22ng范围内线性关系良好,r=0.9997;平均回收率(n=12)为87.81%。结果:本方法测定结果准确,可用于舒血宁注射液的限量检查。Objective ：To establish the limit test of total ginkgolic acid and provide the determinate method and experimental foundation for the related item of Shuxuening injection. Method：Shake the sample with petroleum ether （60 -90 ℃ ）, recover the solvent to dryness, and add methanol to dissolve. The separation was performed on a Inertsil ODS -3 C18 column（4.6 mm × 150 mm,5 μm） ,the mobile phase consisted of acetonitrile and 0. 1% phosphoric acid（85： 10） ,with detection at 300 nm,flow rate at 1 mL ·min^-1. Results：There was a linear relationship with the range of 0. 42 - 2.08 ng, r = 0. 9997 ; The average recovery （ n = 12） was 87.81%. Conclusion： The method is specific, sensitive, accurate, and can be used as the test method of ginkgolic acid.

关 键 词：总银杏酸 白果新酸 舒血宁注射液 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]