小鼠扩张囊胚低渗液处理试验  

Study on Expanded Mouse Blastocysts Treated with Lower Osmatic Solution

作　　者：朱士恩[1] 曾申明[1] 张忠诚[1] 

机构地区：[1]中国农业大学动物科技学院

出　　处：《中国畜牧杂志》1998年第2期9-10,共2页Chinese Journal of Animal Science

摘　　要：为研究解冻后的胚胎膨胀对其发育率的影响，本试验用２０～５０％的低渗ＰＢＳ溶液（即０．２０×、０．２５×、０．３０×及０．５０×），分别将小鼠扩张囊胚在低渗液中处理３０分钟后培养，结果新鲜胚在０．５０×和０．３０×中处理后的发育率分别为１００％和９１％，０．２０×处理组的发育率降低到５３％。解冻后的胚胎直接用低渗液处理，０．５０×组只有８５％继续发育，低于０．２５×组完全不能发育。而解冻后经培养恢复到扩张囊胚后再做低渗处理，０．３０×组仍有９６％继续发育。Expanded mouse blastocyst were treated with lower osmotic solution,20～50% PBS(0.20x,0.25x,0.30x and 0.50x),for 30min to study effects of expanding in thawed embryos on development. Fresh embryos treated with 0.50x,0.30x and 0.20x PBS resulted in development rates of 100%,91% and 53% respectively. Thawde embryos directly treated with 0.50x PBS got developmental rate of 85%,but couldn′t develop with 0.25x PBS. If thawed embryos were re expanded to blastocysts in mKRB firstly,then treated with lower osmatic PBS,the development rates were still 96% in the group treated with 0.30x PBS.

关 键 词：低渗液 膨胀 玻璃化 扩张囊胚 小鼠 

分 类 号：S814.6[农业科学—畜牧学]