黑曲霉糖化酶基因启动子功能鉴定  

Function Characterization of Glucoamylase Gene Promoter from Aspergillus nigerCICIM F0410

作　　者：李昆[1] 李松[1] 牛丹丹[1] 王正祥[1] 

机构地区：[1]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《微生物学杂志》2008年第6期5-9,共5页Journal of Microbiology

基　　金：国家高技术研究发展计划(863计划;2006AA020204)

摘　　要：从糖化酶工业生产菌株Aspergillus nigerCICIM F0410基因组DNA中扩增糖化酶基因启动子(PglaA),并将该启动子替换质粒pRS303K上KmR基因启动子,构建成糖化酶基因启动子功能检测质粒pRS-PglaA-KmR。将pRS-PglaA-KmR转入E.coliJM109中,得到重组菌E.coli(pRS-PglaA-KmR)。通过对重组菌的氨基糖苷磷酸转移酶基因活性检测,表明PglaA在E.coli中具有驱动KmR基因表达的活性。采用不同诱导物进行培养发现,葡萄糖、蔗糖、乳糖、麦芽糖或玉米淀粉,可以不同程度增强PglaA的强度。Glucoamylase gene promoter （ PglaA ） from genome DNA of industrial Aspergillus niger strain CICIM F0410 for producing the enzyme was amplified and replace KmR promoter in plasmid pRS303K with it to construct a glucoamylase gene promoter function testing plasmid pRS-PglaA-Km^R. And the testing plasmid was transferred into E. coli JM109 and obtained recombinant E. coli （pSR-PglaA-Km^R）. Through the examination the activity of amino-glueoside phosphate transferring enzyme of the recombinant bacterium indicated that Pgla^A possessed the activity to drive the genetic expression of Km^R. It was found that culture adopting with different inducers, glucose, sucrose, lactose, maltose, and corn starch, the strength of PglaA was enhanced to different degrees.

关 键 词：黑曲霉 糖化酶基因启动子 功能鉴定 

分 类 号：Q78[生物学—分子生物学]