实时PCR法在慢性前列腺炎病原学诊断中的应用研究  

Application of real-time PCR in the pathogenic diagnosis of chronic prostatitis

作　　者：文东升[1] 杨庆[2] 沈明[3] 

机构地区：[1]湖南省人民医院泌尿外科,长沙410005 [2]浙江省金华市人民医院泌尿外科 [3]昆明医学院第二附属医院

出　　处：《中国男科学杂志》2008年第12期39-43,共5页Chinese Journal of Andrology

摘　　要：目的为研究前列腺细菌感染和慢性前列腺炎之间潜在的关联,本文报告了应用实时PCR方法检测前列腺液(EPS)或按摩前列腺后的尿液(VB3)中的细菌。方法细菌模板DNA取自慢性前列腺炎患者的EPS或VB_3标本。1对通用引物:用于扩增所有已知细菌的核糖体DNA(16SrDNA)。3个荧光探针:一个为检测所有细菌的通用探针(Uniprobe);1个为检测大肠杆菌的特异性探针(Ecprobe);1个为检测金黄色葡萄球菌的特异性探针(SAprobe)。结果应用实时PCR方法检测慢性前列腺炎患者,80个标本中有28个检测到细菌,包括14个为大肠杆菌阳性,6个为金黄色葡萄球菌阳性。结论实时PCR具有能定量、快速、特异等优点,有望成为能对慢性前列腺炎患者EPS或VB_3中的细菌进行定性、定量检测的一种很有前途的方法。Objective To investigate the potential relationship between prostatic bacterial infection and chronic prostatitis （CP）, bacteria in expressed prostate secretions（EPS） or voided bladder 3 （VB3） was detected using real- time PCR. Methods Template DNAs were extracted from EPS or VB3 of patients with chronic prostatitis. A pair of PCR primers were designed for all known bacterial ribosomal DNAs（16SrDNAs）. Three fluorescent probes includ- ing Uniprobe（ all known bacteria）, ECprobe（Escherichia coli） and Saprobe（Staphylococcus aureus） were used in real- time PCR assay. Results Bacteria DNA in 28 samples from patients with chronic prostatitis were found by real-time PCR, including 14 positive samples of E.coli and 6 positive samples of S.aurs. Conclusion Real-time PCR with advantages in quantitative analysis, speed of detection and specificity showed a promising future for the detection of EPS （VB3） bacteria in chronic prostatitis patients.

关 键 词：前列腺炎/微生物学 诊断 聚合酶链反应 

分 类 号：R697.33[医药卫生—泌尿科学] R378[医药卫生—外科学]