辐射诱变甜荞高产突变体过氧化物酶活性及其同工酶的研究  

Study on Peroxidase Activity and Its Isoenzymes of High yield Mutant in Radiation Breeding of Common Buckwheat

作　　者：张小冰[1,2,3] 郝建平[1,2,3] 裴雁曦[1,2,3] 李国柱[1,2,3] 刘晓琴[1,2,3] 

机构地区：[1]太原师范专科学校生化系 [2]山西大学生命科学系 [3]山西农业大学农业生物物理室

出　　处：《华北农学报》1998年第1期71-73,共3页Acta Agriculturae Boreali-Sinica

基　　金：山西省科委资助

摘　　要：甜荞种子经６０Ｃｏ－γ射线照射后从１００００ｒａｄ的剂量组中选出了两个优良高产突变体Ⅱ－４５－１和Ⅲ－３６－１。研究表明，Ⅱ－４５－１、Ⅲ－３６－１的过氧化物酶活性均比对照（未经辐照处理的甜荞种子）高２倍。过氧化物酶同工酶酶谱显示，Ⅱ－４５－１和Ⅲ－３６－１均比对照增加一条Ｒｆ值为０．４３的酶带，减少一条Ｒｆ值为０．８４的酶带。Two fine high yielding mutants (Ⅱ 45 1 and Ⅲ 36 1)of common buckwheat were obtained from 10 000 rad seed radiation by 60 Co γ ray.The results indicates that the peroxidase activity of Ⅱ 45 1 and Ⅲ 36 1 is three times that of unradiated buckwheat.The isoenzymes zymogram of peroxidase shows that both Ⅱ 45 1 and Ⅲ 36 1 have an additional band (Rf=0.43)while a band (Rf=0.84)is missing in comparison with the unradiated buckwheat.

关 键 词：突变体 过氧化物酶 同功酶 荞麦 育种 辐射诱变 

分 类 号：S517.035.2[农业科学—作物学]