鸡舍环境镰孢菌的定量研究  

Quantitative Study on Fusarium in Chicken House Environment

作　　者：王雅玲[1] 彭红艳[1] 王钰卓[2] 李婷婷[1] 胡敏锐[1] 宋莉[1] 郭静[1] 吴迪[1] 

机构地区：[1]大连民族学院生命科学学院,辽宁大连116600 [2]沈阳农业大学,辽宁沈阳110161

出　　处：《安徽农业科学》2008年第36期15900-15901,15903,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(30771584)

摘　　要：[目的]对鸡舍空气和周围基质（饲料、粪便、灰尘、土壤）中镰孢菌（Fusarium）的浓度和分布进行定量研究,为控制从业人员和禽类Fusarium感染及预警提供科学依据。[方法]采用6级Anderson空气微生物采样器和曝皿法采集空气中样本,采用无菌袋随机采取周围基质中样本,进行实验室培养、计数、纯化。[结果]共得到镰孢菌分离株717株,以灰尘中Fusarium的浓度最高（2.8×10^5-1.5×10^6cfu/g,平均为8.8×10^5cfu/g）。[结论]空气中的镰孢菌分布与土壤中和灰尘中镰孢菌相关性较大。[Objective] The aim of this study is to quantitatively analyze the Fusarium concentration and distribution in the air and the surrounding substrates（feed,feces,dust,soil） in the chicken house to provide a scientific basis for the control and early warning of Fusarium infection in poultry and practitioner.[Method] Air samples collected by a 6-stage Anderson air microorganism sampler and exposed culture plates as well as substrate samples gathered by aseptic bags were cultured,counted and purified in the lab.[Result] 717 strains of Fusarium isolates were obtained and the dust had the highest Fusarium concentration among the samples（2.8×10^5-1.5×10^6 cfu/g with an average of 8.8×10^5 cfu/g）.[Conclusion] The Fusarium distribution in the air was in high correlation with those in soil and dust.

关 键 词：镰孢菌 空气 基质 鸡舍 

分 类 号：S831.45[农业科学—畜牧学]