禽霍乱·新城疫二联油乳剂灭活疫苗研究(Ⅲ报)——疫苗对鸭和鹅的安全性及免疫效力试验  

Study on the Inactivated Oil Emulsion Binary Vaccine against Newcastle Disease and Fowl Cholera (Ⅲ)——Safety and Immune Efficacy Test of the Vaccine on Duck and Goose

作　　者：王红琳[1] 杨峻[1] 邵华斌[1] 温国元[1] 艾地云[1] 罗玲[1] 罗青平[1] 张蓉蓉[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,湖北武汉430064

出　　处：《安徽农业科学》2008年第36期15902-15903,共2页Journal of Anhui Agricultural Sciences

基　　金：湖北省科技厅十一五重大攻关项目(2006AA202A05)

摘　　要：[目的]为禽霍乱、新城疫二联油乳剂灭活疫苗免疫鸭和鹅等水禽提供理论依据。[方法]将禽A型多杀性巴氏杆菌接种固体培养基收获的菌液与鸡新城疫病毒弱毒株La Sota接种易感鸡胚收获的感染鸡胚液混合,用甲醛溶液灭活后制备成5批二联油乳剂灭活苗,用于鸭和鹅的安全性及免疫效力试验。[结果]5批二联苗对鸭和鹅的安全性试验表明,免疫鸭和鹅均未出现任何不良反应;免疫效力试验表明,免疫后3周,鸭和鹅的新城疫血凝抑制抗体效价均≥4 log2,新城疫攻毒保护率均为100%,禽霍乱攻毒保护率为66.7%-83.3%。[结论]该二联苗用于免疫鸭和鹅安全可靠,免疫效果良好。[Objective] The aim of this study is to provide theoretical basis for immunizing waterfowls（duck and goose） with the inactivated oil emulsion binary vaccine against Newcastle Disease（ND） and Fowl Cholera（FC）.[Method] Bacterial liquid from solid culture media inoculated avian Pasteurella multocida（APM） type A and allantoic fluid from embryonic eggs infected with Newcastle Disease Virus（NDV） attenuated strain La Sota were mixed and inactivated by formalin to prepare 5 batches of inactivated oil emulsion binary vaccine,which were then used for the safety and immune efficacy test on duck and goose.[Result] Immunized ducks and geese didn＇t performed any adverse reactions in the safety test of the 5 batches of vaccine;the immune efficacy test showed that ND-HI antibody titers of ducks and geese were no less than 4 log2 three weeks after inoculation,and the protection rates against NDV and APM were 100% and 66.7%-83.3%,respectively.[Conclusion] The binary vaccine against ND and FC is safe and reliable for duck and goose,and can provide sufficient immunity protection against ND and FC.

关 键 词：禽霍乱 新城疫 二联苗 安全性 免疫效力 

分 类 号：S859.5[农业科学—临床兽医学]