甘薯脱毒技术及增产效果研究  

PRODUCE TECHNIQUES AND PRACTICE OF VIRUS-FREE SWEET POTATO

作　　者：杨崇良[1] 尚佑芬[1] 赵玖华[1] 李长松[1] 

机构地区：[1]山东省农业科学院植物保护研究所,济南250100

出　　处：《植物保护学报》1998年第1期51-55,共5页Journal of Plant Protection

基　　金：山东省计划委员会"八五"攻关

摘　　要：作者于1988～1995年研究了甘薯病毒及甘薯脱毒技术,明确了侵染山东甘薯的主要病毒种类是甘薯羽状斑驳病毒和甘薯潜隐病毒,在国内首次分离出侵染甘薯的烟草花叶病毒,探明了其生物学特性。筛选出适合山东甘薯茎尖培养基的最佳激素配比、浓度、pH值。探索了脱毒薯的增产机理和增产效果,提出了组织培养、茎尖苗检测、脱毒薯速繁与推广应用的配套技术规程。培养出了徐薯18等12个品种的脱毒苗。平均增产42.9％,出干率提高1～3个百分点,对甘薯黑痣病、黑斑病、茎线虫病的防效明显。The sweet potato viruses and the techniques to get virus-free sweet potato was studied in 1988 - 1995. It was clarified that the main sweet potato viruses were SPFMV and SPLV in Shandong Province. TMV infecting sweet potato was isolated for the first time in China. The biological features were studied. The medium for culturing virus-free sweet potato was screened. The mechanism of yield increase of virus-free sweet potato and the effect of yield increase were investigated. A complete technique process for application of virus-free sweet potato including apical meristem culture, virus test of regenerated plants, virus-free sweet potato propagation was proposed. Twelve cultivars including Xushu 18 were cultivated. The yield of virus-free sweet potato increased 42. 9% in average compared to the control, which was infected by the virus. The infection of Monilochaetes infuscans, Ceratocystis fimbreata and Prathlenchus coffeac decreased 90% in comparison with the control.

关 键 词：甘薯 病毒病 毒原鉴定 脱毒 病毒检测 茎尖苗 

分 类 号：S531.034[农业科学—作物学]