CYP450抑制剂SKF525A对大鼠脏器和血管CYP2J3 mRNA表达和EET含量的影响  

Influence of the CYP450 Inhibitor Injected through Abdominal Cavity on CYP2J3/EETs System in Rat

作　　者：王晶[1] 芦玲巧[1] 王红霞[1] 常静[1] 曾翔俊[1] 张立克[1] 

机构地区：[1]首都医科大学病理生理学教研室,北京100069

出　　处：《微循环学杂志》2009年第1期4-6,共3页Chinese Journal of Microcirculation

基　　金：北京市自然科学基金资助项目(7062007)

摘　　要：目的:观察腹腔注射细胞色素P450(CYP450)抑制剂SKF525A后,大鼠心、肝、肺、肾、胸主动脉组织CYP2J3mRNA表达及11,12-二十碳三烯酸(11,12-EET)含量的变化。方法:将24只雄性Wistar大鼠随机分为对照组(Sham组)和抑制剂组(SKF组),对照组腹腔注射生理盐水,SKF组腹腔注射SKF525A,分别在注射后15天及30天取材,采用半定量RT-PCR方法检测大鼠心、肝、肺、肾及胸主动脉组织CYP2J3mRNA的表达变化,采用高效液相色谱方法测定上述组织11,12-EET含量变化。结果:与15天Sham组相比,15天SKF组各种组织CYP2J3的mR-NA表达及11,12-EET含量均降低(P<0.05,P<0.01),而30天SKF组与30天Sham组相比,各组织CYP2J3的mRNA表达及11,12-EET含量均有升高(均P<0.05,P<0.01)。结论:CYP450抑制剂SKF525A可有效抑制CYP2J3在各种组织中的mRNA表达及11,12-EET的生成,但这种抑制作用会随着停药时间的延长而逐渐降低或消失。Objective:To detect the changes of CYP2J3 gene expression and contents of 11,12-EET in rat tissues after injecting inhibitor of CYP450.Method:The rat models was made by peritoneal inject SKF525A,an inhibitor of CYP450.The rats were divided into Sham group and SKF group randomly.The tissues were taken at the 15 day and the 30 days after injection.RT-PCR was used to assess the gene expression of CYP2J3 mRNA level in heart,liver,lung,kidney and aorta thoracalis.The high pressure liquid chromatography(HPLC)was used to detect contents of 11,12-EET in these tissues.Results:Compared with Sham 15 d group,the expression of CYP2J3 mRNA and the content of 11,12-EET were significantly decreased in SKF 15 d group.But compared with Sham 30d group,the expression of CYP2J3 mRNA and the content of 11,12-EET were significantly increased in SKF 30 d group.Conclusion:As an inhibitor of CYP450,SKF525A could inhibit the expression of CYP2J3 mRNA and contents of 11,12-EET relative material effectively.But the inhibited action of SKF525A was decreased with time.

关 键 词：抗心血管疾病 P450抑制剂 CYP450 MRNA表达 EET 大鼠 环氧-二十碳三烯酸 细胞色素P450 

分 类 号：R972[医药卫生—药品] R978.5[医药卫生—药学]