芦丁-镧-核酸体系的荧光增强效应及其分析应用的研究  

Fluorescence enhancement of rutin-La^(3+)-nucleic acid system and its analytical application

作　　者：郭蒙[1] 杨景和[2] 吴霞[2] 丁红红[2] 郭勤 

机构地区：[1]潍坊学院化学化工系,山东潍坊261061 [2]山东大学化学与化工学院,山东济南250100 [3]山东信息技术学院图书馆,山东潍坊261061

出　　处：《化学试剂》2009年第2期81-85,共5页Chemical Reagents

基　　金：国家自然科学基金资助项目(20575035);山东省自然科学基金资助项目(Y2003B02);潍坊学院自然科学基金资助项目(2008Z04)

摘　　要：核酸能增强芦丁-La3+体系荧光强度。在最佳实验条件下,fsDNA和yRNA的浓度分别在0.08～10和0.1～9mg/L范围内与体系的荧光强度呈良好的线性关系,其检出限分别为0.08和0.054mg/L。该法已用于合成样品的测定,结果令人满意。另外,还研究了体系的相互作用和荧光的增强机理。Experiments indicate that nucleic acid could enhance the fluorescence intensity of La^3 ＋ -rutin system. Under the optimum conditions, the fluorescence intensity of the system is in proportion to the concentration of nucleic acids in the range of 0.08 - 10 mg/L for fsDNA and 0.1 - 9 mg/L for yRNA, and their detection limits are 0,08 mg/L for fsDNA, and 0.054 mg/L for yRNA, respectively. The synthetic samples were satisfactorily determined. In addition, the mechanism for the interaction and fluorescence enhancement of the system was studied.

关 键 词：芦丁 核酸 镧(Ⅲ) 荧光增强效应 

分 类 号：O657.3[理学—分析化学]