RP-HPLC测定脑得生软胶囊中三七皂苷R_1、人参皂苷Rb_1及人参皂苷Rg_1含量  

Determination of notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 in Compound Naodesheng Soft Capsule by HPLC

作　　者：宋恬[1] 李桥[1] 何再安[1] 王光忠[1] 刘焱文[1] 

机构地区：[1]湖北中医学院,"中药资源与中药复方"省部共建教育部重点实验室,湖北武汉430061

出　　处：《中成药》2009年第2期237-240,共4页Chinese Traditional Patent Medicine

基　　金：武汉市科技局专项资金资助项目(20056005130001)

摘　　要：目的:用高效液相色谱梯度洗脱法同时测定脑得生软胶囊(三七、川芎、葛根、红花、山楂)中三七皂苷R1、人参皂苷Rb1和人参皂苷Rg13种皂苷的含量,为制定该制剂质量标准中含量测定方法及限度提供依据。方法:用美国热电公司HypersilGOLD C18柱(4.6mm×250mm,5μm),乙腈-水梯度洗脱,0～25min(18∶82～40∶60),平衡10min,流速:1.0mL/min,检测波长:203nm。结果:该方法回收率R1:98.8%(RSD=0.67%),Rb1:103.9%(RSD=1.66%),Rg1:102.5%(RSD=1.17%)。结论:HPLC梯度洗脱法能将多种皂苷很好地分离检测,提高了时效,减少了误差,结果表明该方法准确可靠,重现性好,结果稳定。AIM： To establish RP-HPLC method for simultaneously determining three main medicinal constituent, notoginsenoside R1 and ginsenoside Rg1 , Rb1 in Compound Naodesheng Soft Capsule （Radix et Rhizoma Notoginseng, Rhizoma Chuanxiong, Radix Puerariae Lobatae, Flos Carthami, Fructns Crataegi ）. METHODS： The HPLC system consisted of Hpersil ODS column （250 mm× 4.6 mm, id5 μm）, a mobile phase of acetonitrile-water 0-25 min of （18：82 -40： 60） gradient elution. Equilibrium time was 10 min, column temperature was set at 30 ℃ and the wavelength of detector was set at 203 nm. RESULTS ： The average recoveries of R1, Rg1 and Rb1 were 98.8% （RSD = 0.67% ）, 103.9% （RSD = 1.66% ） and 102.5% （RSD = 1. 17% ）, respectively. CONCLUSION： The method is rapid, accurate, sensitive and reliable.

关 键 词：PR-HPLC 复方脑得生软胶囊 人参皂苷RG 人参皂苷RBL 三七皂苷R1 

分 类 号：R927.2[医药卫生—药学]