“葛根芩连汤”两种配伍比例的化学药效组分比较分析  

Comparison of active components alignment from two kinds of compatibility proportion in Puerariae Decoctions

作　　者：崔向微[1] 张贵君[1] 李慧[1] 张春晖[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中成药》2009年第2期263-266,共4页Chinese Traditional Patent Medicine

基　　金：国家教育部科学技术研究重点项目(00167)

摘　　要：目的:分析两种配伍比例的葛根芩连汤中化学药效组分的变化规律。方法:反相高效液相色谱法(RP-HPLC)。结果:葛根-黄芩-黄连-炙甘草在5∶3∶3∶2的配伍关系下,葛根素-黄豆苷-黄芩苷-小檗碱-巴马汀-药根碱-甘草酸-甘草苷的含量分别为:2.48%、0.101%、0.614%、0.155%、0.048%、0.094%、0.154%、0.283%,比例关系为51.7∶2.1∶12.8∶3.2∶1∶2∶3.2∶5.9;在8∶3∶3∶2的配伍关系下,含量分别为:1.67%、0.143%、0.36%、0.085%、0.031%、0.048%、0.126%、0.174%,比例关系为54.4∶4.7∶11.7∶2.6∶1∶1.5∶4.1∶5.7。结论:葛根-黄芩-黄连-炙甘草在8∶3∶3∶2配伍比例时,只是葛根的药效成分比例相对增高,其余化学药效组分的比例无显著性差异,说明化学药效组分的含量与配伍组分比例及溶解度有关。AIM： To analyze the changes in the active components alignment of Puerariae Decoctions between its two different compatibility proportion. METHODS： Reversed phase high-performance liquid chromatography （RP-HPLC） was applied. RESULTS ： When the compatibility ratio of Radix Puerariae Lobatea, Radix Scutellari- ae, Rhizoma Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 5： 3： 3： 2, the contents of the eight drug active components alignment were 2.48% puerarin, 0. 101% daidzin, 0. 614% baicalin, 0. 155% berberine, 0.048% palmatine, 0.094% jateorhizine, 0. 154% glycyrrhizic acid, 0. 283% liquiritin, and the propor- tional relationship of compatibility was 51.7： 2.1： 12.8： 3.2： 1： 2： 3.2： 5.9. When the compatibility ratio of Radix puerariae lobatae, Radix scutellariae, Radix Coptidis and Radix et Rhizoma Glycyrrhizae Praeparata cum melle was 8： 3： 3： 2, the proportion of contents of the eight active components alignment were 1.67% , 0. 143%, 0.36%, 0. 085%, 0.031%, 0.048%, 0. 126%, 0. 174%. And the proportional relationship of compatibility was 54.4：4.7 ： 11.7： 2.6： 1： 1.5： 4.1： 5.7. CONCLUSION： When the compatibility percentage consists of 8： 3： 3： 2, only the puerarin content relatively increases. The active components alignment of the rest have no significant difference. This illustrates the contents of the active components alignment of puerariae decoction is related to the compatibility percentage and herb solubility.

关 键 词：葛根芩连汤 RP-HPLC 化学药效组分 

分 类 号：R284.1[医药卫生—中药学]