RP-HPLC测定家兔血浆中的替普瑞酮  

Determination of Teprenone in rabbit plasma by RP-HPLC

作　　者：唐丽红[1] 李婧[1,2] 张丹[1] 毛惠英[1] 吴意红[1] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]西南民族大学,四川成都610041

出　　处：《华西药学杂志》2009年第1期64-65,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用RP-HPLC法,测定兔血浆中的替普瑞酮。方法血浆样品中加入丁烯二酸二异辛酯和NaH2PO4溶液(0.05mol·L-1,pH2.5),用叔丁醚振荡萃取后测定。色谱柱为LunaC8柱(150mm×4.6mm,5μm),流动相为乙腈-水(92:8),流速1.0m.lmin-1,柱温25℃,检测波长205nm。结果替普瑞酮0.086～3.440μg.ml-1线性关系良好(r=0.9991),平均方法回收率为98.25%,日内和日间RSD均小于10%。结论所建方法简便、灵敏、准确、重复性好,适用于替普瑞酮的药物动力学研究。OBJECTIVE A simple RP - HPLC method was established for determination of Teprenone in rabbit plasma. METHODS Internal standard diisooctyl maleate and NaH2PO4 （0. 05 mol·L-1 ,pH2. 5）were added to plasma sample, then the sample was extracted with tertbutylmethylether and the extract was determined on a Cs analytical column（ 150 mm× 4. 6 mm, 5 μm）. The experi- ment was performed with mobile phase of aeetonitrile - water （ 92 ： 8 ） at a flow rate of 1.0 ml·min-1and detected at 205 nm. RESULTS The linear range was 0.086 - 3. 440μg· ml- 1 （ r = 0. 9991 ）. The analytical recovery was 98.25 %, and the RSD of within -day and between -day RSD was less than 10%. CONCLUSION This method is simple, sensitive, accurate and reproducible. It is applicable for pharmacokinetic study of Teprenone.

关 键 词：替普瑞酮 反相高效液相色谱法 兔血浆 

分 类 号：R917[医药卫生—药物分析学]