RP-HPLC测定家兔血浆中的儿茶素和表儿茶素  

Determination of the catechin and epicatechin in rabbit plasma by RP-HPLC

作　　者：毛惠英[1] 张丹[1] 李鑫楠[1] 唐丽红[1] 吴意红[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2009年第1期66-67,共2页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用RP—HPLC法测定家兔血浆中的儿茶素和表儿茶素。方法色谱柱为Hypersi lC18柱（250mm×4．6mm，10μm），测定儿茶素和表儿茶的流动相分别为乙腈-水-三乙胺（8：92：0．3、11：89：0．3），流速均为1．0ml·min-1，检测波长分别为206、204nm，采用甲醇直接沉淀蛋白，取上清液进样。结果家兔血浆中内源性成分测定无干扰；儿茶素0．68—6．50μg·ml-1呈良好的线性关系（r=0．9994），平均方法回收率为94．7％，日内和日间RSD分别为2．72％和2．84％；表儿茶素0．25～9．92μg·ml-1呈良好的线性关系（r=0．9991），平均方法回收率为99．5％，日内和日间RSD分别为2．52％和5．92％。结论所建方法简便，灵敏度较高，结果准确，可为儿茶药材及含儿茶中药制剂的体内研究提供参考。OBJECTIVE To establish a sensitive and reliable RP - HPLC method for detemination of the catechin and epicatechin in rabbits plasma. METHODS Concentrations of catechin and epieatechin in rabbits plasma were measured by RP - HPLC after pre- treatment with methanol solution to precipitate the protein. Separation and determination were achieved on a Hypersil CIE column（ 250 mm× 4. 6 mm, 10 μm） with the mobile phase consisted of acetonitrile - water - triethylamine （ 8 ： 92 ： 0. 3 ） for cateehin and acetoni- trile- water- triethylamine （ 11 ： 89 ： 0. 3 ） for epicatechin. The UV detector wavelengths were set at 206nm and 204nm, the fluid speeds both were 1.0 ml·min - 1. RESULTS There was no interference found during the determination. Good linear relationship of cat- eehin and epieatechin were separately obtained in the range of 0. 68 to 6. 50 μg·ml-1（ r = 0. 9994） and 0. 25 to 9. 92μg·ml-1 （ r = 0. 9991 ）, the mean recoveries were 94.7% and 99. 5% , the within - day RSD were 2. 72% and 2.52%, and the between - day RSD were 2. 84% and 5.92%. CONCLUSION The methods are simple, sensitive, accurate with a wide linear, and suitable for the inter- nal study of Cateebu and Chinese materia mediea preparation containting catechin.

关 键 词：高效液相色谱法 JL茶素 表儿茶素 血药浓度 

分 类 号：R917[医药卫生—药物分析学]