四川省两县区乙型肝炎病毒基因型、血清亚型分析  

ANALYSIS OF GENOTYPES AND SUBTYPES OF HEPATITIS B VIRUS IN TWO COUNTIES OF SICHUAN

作　　者：童文彬[1] 孙莉[1] 何吉兰[1] 李天舒[1] 杜飞[1] 汪洋[2] 熊朝阳[3] 

机构地区：[1]四川省疾病预防控制中心,成都610041 [2]成都市青羊区疾病预防控制中心 [3]仁寿县疾病预防控制中心

出　　处：《现代预防医学》2009年第4期763-765,共3页Modern Preventive Medicine

摘　　要：[目的]了解四川省两县区乙型肝炎病毒的基因型和血清型。[方法]从HBsAg、HBeAg均阳性的血清中提取病毒DNA,经多聚酶链反应和S基因核苷酸片段测序后,用进化树和推导氨基酸序列确定基因型和血清亚型。[结果]49份血清标本中,含w亚决定簇的均为B基因型,含r亚决定簇的均为C基因型;其中B基因型/adw2血清亚型31份,B基因型/ayw1血清亚型1份,B基因型/adw3血清亚型1份,C基因型/adrq+血清亚型14份,C基因型/ayr血清亚型2份。[结论]两县区乙型肝炎病毒以B基因型/adw2血清亚型和C基因型/adrq+血清亚型为主,B基因型/adw2血清亚型占优势;同时存在少见的B基因型/adw3血清亚型。[Objective] To investigate genotype and subtype of hepatitis B virus in 2 counties （districts） of Siehuan province. [Methods] Vires DNA were extracted from samples both positive for HBsAg and HBeAg. After polymerase reaction （PCR） and sequencing of partial S gene, genotypes and subtypes were analysized by phylogenetie analysis and deduced amino acid sequences, respectively. [Results] Of 49 samples, those with w subdeterminant and those with r subdeterminant belonged exclusively to genotype B and genotype C, respectively; 31 samples were genotype B/subtype adw2, 1 was genotype B/ subtype aywl, 1 was genotype B/subtype adw3, 14 were genotype C/subtype adrq＋, and 2 were genotype C/subtype ayr. [Conclusion]C, enotype B/subtype adw2 and genotype C/subtype adrq＋ accounted for most proportion, and genotype B/subtype adw2 predominated in 2 counties （districts） ; rare genotype B/subtype adw3 also existed.

关 键 词：乙型肝炎病毒 基因型 血清型 

分 类 号：R512.62[医药卫生—内科学]