七叶皂苷钠对P388小鼠白血病细胞的体内外增殖抑制作用  

In vitro and in vivo anti-proliferative activity of aescin in P388 murine leukemia cells

作　　者：竺佳[1] 牛泱平[1] 徐佳[1] 邱燕燕[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310032

出　　处：《浙江工业大学学报》2009年第2期123-125,130,共4页Journal of Zhejiang University of Technology

基　　金：浙江省自然基金资助项目(Y205636);杭州市科技局项目(20052431B06)

摘　　要：探讨了β-七叶皂苷钠对P388小鼠急性淋巴细胞白血病细胞的体内外增殖抑制作用.采用MTT法和建立P388可移植性白血病模型实验治疗方法观察七叶皂苷钠对小鼠急性淋巴细胞白血病P388体外增殖抑制作用,对白血病移植模型小鼠生命的延长率,以及与化疗药阿霉素的协同作用效果.七叶皂苷钠对P388细胞具有明显的体外增殖抑制作用,作用呈时间和剂量依赖性;中、高剂量的七叶皂苷钠对体内白血病模型具有一定的治疗效果,明显延长荷瘤小鼠的生命期(P<0.01),并与常规化疗药阿霉素具有明显的协同增效作用.结论实验结果初步提示七叶皂苷钠能有效抑制P388细胞体内外增殖,可为其临床应用于白血病的治疗提供实验依据.Objective： To explore the in vitro and in vivo anti-proliferative activities of aescin in P388 murine leukemia. Methods： MTT assay was used to determine the anti-proliferative effect in vitro; leukemia animal models were used to test the anti-leukemic effect of aescin or aescin combined with doxorubicin in vivo. Results： aescin elicited a potent suppression of P388 proliferation with an IC50 of 23 μg/ml at 72h in vitro ; In vivo study, span of P388-bearing mice significantly （P〈0. 01）, and the mice therapy using aescin and doxorubicin survived longer than those monotherapy（P〈0.01）. Conclusion. Aescin might be applied in leuke aescin prolonged the lifetreated with combination treated with doxorubicin mia treatment.

关 键 词：七叶皂苷钠 P388急性淋巴白血病细胞 细胞增殖 体内外 

分 类 号：R965.1[医药卫生—药理学]