干姜挥发油提取优化及GC-MS图谱研究  

Studies on volatile oil extraction by orthogonal design and GC-MS analysis for Rhizoma Zingiberis

作　　者：李翔[1,2] 吴豪[1] 朱东亮[1] 柴逸峰[1] 娄子洋[3] 

机构地区：[1]第二军医大学药学院药物分析教研室,上海200433 [2]北京军区疾病预防控制中心,北京100042 [3]第二军医大学药学院分析测试中心,上海200433

出　　处：《药学实践杂志》2009年第1期46-48,50,共4页Journal of Pharmaceutical Practice

摘　　要：目的:优化干姜挥发油提取工艺,同时建立干姜挥发油GC-MS指纹图谱。方法:以挥发油得率为指标,采用正交设计,对加水量、超声时间、浸泡时间和提取时间进行优化,并采集GC-MS图谱。结果:确定加17倍量水,超声35 min,浸泡40 min,再提取8 h的效果最优,挥发油得率分别为1.89%,GC-MS图谱稳定、重复。结论:干姜挥发油经过提取优化可以保证得率,获得的GC-MS图谱稳定可靠,并鉴定出的49种化合物。Objective： To optimize the extraction methods and GC-MS analysis of volatile oil in Rhizoma Zingiberis. Methods： The distilled water volume, ultrasonic time, soaking time and extraction time were optimized by orthogonal design and GC-MS chromatogram was obtained. Results：The yield of volatile oil was 1.89% by orthogonal design. Also, a stable, reliable chromatogram was obtained under optimized GC-MS conditions. Conclusion：The design can be applied for optimizing the extraction condition of volatile oil in Rhizoma Zingiberis and 49 compounds were identified by GC-MS analysis.

关 键 词：干姜 挥发油 正交设计 GC-MS 

分 类 号：R284[医药卫生—中药学]