HPLC法测定委陵菜中黄酮类成分的含量  

Determination of flavonoids in Potentilla chinensis ser by HPLC

作　　者：申东月[1,2] 朴淑娟[2] 林厚文[2] 汤真[3] 张恒弼[3] 

机构地区：[1]延边大学医学部,吉林延吉133000 [2]第二军医大学长征医院药学部,上海200003 [3]中国人民解放军第208医院,吉林长春130061

出　　处：《药学实践杂志》2009年第1期63-65,共3页Journal of Pharmaceutical Practice

基　　金：上海-SK研究与发展基金(2003010-t)

摘　　要：目的：建立HPLC法测定委陵菜中槲皮素、翻白叶苷A和芹菜素含量的方法。方法：色谱柱为ZORBAXSB—C18色谱柱（4．6mm×150mm，5μm）；流动相为甲醇-0．3％磷酸水溶液（51：49）；检测波长为360nm；流速为0．9mL／min。结果：槲皮素、翻白叶苷A和芹菜素的进样量分别在0．72～10．08μg／mL、0．86～12．04μg／mL和0．54～7．56μg／mL范围内与其峰面积呈良好的线性关系，线性系数均为0．9999，平均回收率分别为95．37％、96．87％、97．66％，RSD分别为1．79％、1．63％、2．08％，药材中三中黄酮的含量分别为100．0μg／g、323．0μg/g和55．0μg／g；结论：该方法快速、简便、可靠，适用于委陵菜中黄酮类成分的含量测定。Objective： To develop a HPLC method to determine the content of 3 flavonoids in Potentilla chinensis ser. Methods： ZORBAX SB-C18 column （4.6 mm× 150 mm, 5 μm） was used, and the mobile phase was methanol-0.3% Phosphoric acid （51 ： 49 ） with the flow rate of 0.9 mL/min and the detection wanelength at 360 nm. Results ： The linear ranges of quercetin, potengriffioside A and apigenin were 0.72 - 10.08 μg/mL, 0.86 - 12.04 μg/mL and 0.54 - 7.56 μg/mL, respectively （ r = 0. 999 9） ; the average recovery was 95.37% , 96.87% and 97.66% , the RSD was 1.79% , 1.63% and 2.08% , respectively; the content of these three flavonoids in the herb was 100 μg/g,323 μg/g and 55 μg/g, respectively. Conclusion：This method is fast, and reliable convenient, which refers to the assay of flavanoids in Potentilla chinensis.

关 键 词：HPLC 委陵菜 槲皮素 翻白叶苷A 芹菜素 

分 类 号：R284[医药卫生—中药学]