犬附红细胞体病PCR检测方法的建立及应用  

Establishment and Application of PCR Technique for Detecting Dog Eperythrozoon

作　　者：张伟[1] 刁有祥[1] 臧大鹏[1] 杜爱庆[1] 张瑞平[1] 马艳芳[1] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018

出　　处：《西南师范大学学报（自然科学版）》2009年第1期67-71,共5页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：山东省重大科技攻关资助项目(011020102)

摘　　要：根据Genbank中已经发表的犬附红细胞体16sRNA基因序列设计合成一对特异性引物,通过聚合酶链式反应(PCR)扩增出一条与目的片段大小一致,约630 bp的基因片段,建立了PCR快速检测方法.以建立的PCR检测方法及瑞氏、姬姆萨、吖啶橙染色法为基础对20例犬附红细胞体病例进行检测,比较四种方法的检出率.结果表明:建立的PCR检测方法的检出率略高于吖啶橙染色,明显高于瑞氏、姬姆萨染色,进而证明建立的PCR检测方法可用于犬附红细胞体的快速诊断和流行病学调查.According to the sequence of the published gene, a pair of primers were designed for amplifying the 630bp fragments in PCR. One method was set up for detecting E. perekropvi. The detection rates of staining methods with Wright, Giemsa and acridine stains were complared in search for a reliable method to detect the E. perekropvi. The result showed that the positive rate of PCR was higher than acridine stains, much more higher than Wright, Giems stain, indicating that the PCR assay was better than Wright, Giemsa and acridine stains methods in diagnosis and epidemic investigation.

关 键 词：犬附红细胞体 聚合酶链式反应(PCR) 瑞氏染色 姬姆萨染色 吖啶橙染色 

分 类 号：S858.292[农业科学—临床兽医学]