双黄连抑制流感病毒甲_1型基因的研究  

Study on Shuanghuanglian inhibiting effect of influenza virus A1 gene

作　　者：孙坚[1] 王农荣[1] 杨斌[1] 何士勤[1] 

机构地区：[1]南昌大学第四附属医院,江西330003

出　　处：《临床肺科杂志》2009年第3期298-300,共3页Journal of Clinical Pulmonary Medicine

基　　金：江西省自然科学基金资助项目

摘　　要：目的通过对实时荧光定量RT-PCR检测流感病毒甲1型多聚酶蛋白(PA)基因,了解双黄连作用病毒的效应。方法采用实时荧光定量RT-PCR法标准曲线测药物抑制甲1型PA病毒基因效应。结果以实时荧光RT-PCR法,重复测7次取平均值,对照组病毒基因为3.62×106±0.90拷贝数/ug,试验组病毒基因为4.57×105±1.23拷贝数/mg。发现病毒对照组基因明显高于试验组(P<0.001)。结论提示双黄连可抑制流感病毒甲1基因。Objective To establish a real - time fluorogenic quantitative RT-PCR method for detecting the expression of PA gene of influenza virus A1 gene and to investigate the iuhibitore effect of Shuanghuanglian. Methods Real-time fluorogenic quantitative RT- PCR （FQ-RT-PCR） was set up to examine the specific expression of test group and virus control group PA gene in virus. Results After 7 duplicate tests with the FQ-RT-PCR. method, the average level of the virus control group was 3.62 × 10^6 ±0.90 copies/μg RNA in virus and 4. 57 × 10^5±1.23 copies/μg RNA in virus test group. Virus control group was significantly higher than the test group （ P 〈 0. 001 ）. Conclusion This showe that Shuanghuanglian could inhibit PA gene expression of influenza virus A1.

关 键 词：双黄连 流感病毒 实时荧光定量RT-PCR 基因 

分 类 号：R285.5[医药卫生—中药学]