实时荧光定量聚合酶链反应检测支气管肺泡灌洗液中结核分枝杆菌-DNA对肺结核的诊断价值  

Clinical diagnostic value of quantification of Mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid by real-time fluorescence polymerase chain reaction

作　　者：张晶[1] 邓群益[1] 高扬[1] 吴驰[1] 

机构地区：[1]深圳市东湖医院,518000

出　　处：《临床肺科杂志》2009年第3期336-337,共2页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨实时荧光定量聚合酶链反应法(FQ-PCR)检测支气管肺泡灌洗液(BALF)中结核分枝杆菌-DNA(TB-DNA)对肺结核的诊断价值。方法肺结核52例(菌阳20例,菌阴32例),肺炎35例,采用FQ-PCR法检测其支气管肺泡灌洗液中TB-DNA水平。结果52例肺结核组的阳性率为65.4%(菌阳19例,菌阴15例),35例非肺结核组的阳性率为5.7%,经统计学比较,FQ-PCR法检出结核杆菌阳性率显著高于痰涂片抗酸染色和培养法(P均<0.05),FQ-PCR法特异性高。结论FQ-PCR法检测BALF中TB-DNA为痰涂片阴性及无痰患者提供良好的诊断依据。Objective To evaluate the value of real-time fluorescence polymerase chain reaction （FQ-PCR） for detection of Mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid. Methods Mycobaeterium tuberculosis DNA in bronehoalveolar lavage fluid obtained from 52 patients with tuberculosis pulmonary disease and 35 patients with non-tuberculosis pulmonary disease were detected by FQ-PCR. The results were statistically analyzed. Results The positive rate of Mycobaeterium tuberculosis detected with FQ-PCR was higher than that with smear acid-fast and culture of organism. The positive rate of non - tuberculosis was only 5.7%. The specificity was higher. Conclusion The detection of TB-DNA in BALF by FQ-PCR may provide valuable information for the diagnosis of smear negative tuberculosis patients.

关 键 词：结核分枝杆菌 诊断 实时荧光定量聚合酶链反应 

分 类 号：R450[医药卫生—治疗学] R521[医药卫生—临床医学]