Toll样受体在胆道闭锁肝脏中的表达  

The expression of Toll-like receptors in livers of patients with biliatry atresia

作　　者：杨瑛[1] 汤绍涛[1] 曹国庆[1] 赵志祥[1] 董宁[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院小儿外科,武汉430022

出　　处：《中华小儿外科杂志》2009年第2期72-75,共4页Chinese Journal of Pediatric Surgery

基　　金：国家十一五科技支撑攻关课题（2006BA105A06）;湖北省卫生厅资助项目（2005JX2B19）;湖北省自然科学基金（2006ABA159）

摘　　要：目的 检测胆道闭锁（biliary atresia，BA）患儿肝脏组织中，Toll样受体（TLRs）的表达情况，探讨其与胆道闭锁发生的关系。方法应用RT-PCR和Westenblot方法检测13例BA患儿、7例胆总管扩张（CC）患儿和8例正常对照儿童（CO）肝脏组织中TLRsmRNA和蛋白的表达。结果BA患儿肝脏组织TLR7表达明显高于CC组患儿和CO组患儿（P＜0．05）；而TLR3的表达在BA组患儿和CC组患儿之间差异无统计学意义，略高于C0组患儿（P〉0．05）。TLR4的表达在BA组患儿和CC组患儿之间差异无统计学意义（P〉0．05），但高于CO组患儿（P〈0．05）。结论胆道闭锁患儿肝组织中TLR3、TLR7、TLR4表达异常，可能在胆道闭锁胆管损伤中发挥作用。Objective To explore the expression of Toll-like receptors in livers ot patients with biliary atresia（BA） and to explore the relationship between Toll-like receptors and BA. Methods Expression of mRNA and protein of Toll-like receptors in livers of 13 patients with BA, 7 with choledochal cyst （CC） and eight normal control（CO） children was detected by means of RT-PCR and western blot. Results The expression of TLR7 was significantly higher in patients with BA than in those with CC and CO（P〈0. 05） TLR3 expression was a little higher in patients with BA than in CO（P〉0. 05）, and no statistically significant difference could be noted between patients with BA and CC （P〉 0. 05） there was no statistically significant difference of the expression of TLR4 between patients with BA and CC（P〉0. 05）, while the expression of TLR4 was significantly higher in patients with BA than in CO（P〈0. 05）. Conclusions There is abnormal expression of Toll-like receptors in the livers of patients with BA. A possible explanation is that TLR3, TLR7, TLR4 may play important roles in the lesion of biliary tract in patients with BA.

关 键 词：胆道闭锁 TOLL样受体 聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]