牵张应力对小儿体外骨髓间充质干细胞向成骨、成脂肪分化的相关性研究  

Investigation on the correlation between mechanical strain and the differentiation of mesenchymal stem cells of children＇s bone marrow to osteoblasts and adipocytes in vitro

作　　者：黎润光[1] 邵景范[1] 魏明发[1] 洪振亚[2] 杨勇[3] 杨小进[1] 周学峰[1] 陈超[1] 冯杰雄[1] 袁继炎[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院小儿外科,武汉430330 [2]华中科技大学同济医学院附属同济医院血液科 [3]华中科技大学同济医学院附属同济医院骨科

出　　处：《中华小儿外科杂志》2009年第2期110-113,共4页Chinese Journal of Pediatric Surgery

基　　金：教育部回国人员基金项目（013456）

摘　　要：目的 探讨周期性机械应力对小儿骨髓间充质干细胞（MSCs）分化方向的影响。方法利用自行建立的机械牵张应力装置，对体外MSCs加载力学信号，并复合成脂肪诱导剂，选用化学染色、RT-PRC、免疫组化、Western Blot的方法检测MSCs干预后成骨指标（OC、BMP-2、Ⅰ型胶原）和成脂肪分化的指标（PPARγ2、脂滴）。结果MSCs受力后，细胞OCmRNA表达较对照组明显增高，而且与干预时间相关；干预7d后，细胞内BMP-2、Ⅰ型胶原蛋白表达较对照组明显增强；MSCs在成脂肪诱导过程中，接受应力后细胞脂滴的出现和PPART-2mRNA的表达均明显下降。结论适当的应力促进MSCs向成骨方向分化，抑制其向脂肪方向分化。Objective To study the correlation between mechanical strain and the differentiation of mesenchymal stem cells（MSCs） of children＇s bone marrow to osteoblasts and adipoeytes in vitro. Methods The MSCs were isolated and primary cultured. After 3rd - 6th passage of MSCs,mechanical stretch,and （or） condition medium was perform on MSCs. Cytochemical staining,RT-PCR,immunocy toehemical staining and Western Blot were applied to measure the markers of osteoblasts （OC,BMP-2, Ⅰ-col）and the markers of adipocytes （lipid droplets, PPARγ-2）. Results After mechanical stretch treatment, the level of intracellular OC rnRNA was higher than that of the control group, which showed the correlation between the intracellular OC mRNA and the stimulating period. After stimulating the MSCs for 7days, the expression of BMP-2 and Ⅰ-col was significantly higher than that of the control group. In the process of induction of MSCs to adipocytes, the mechanical stretched cells had less intracellular lipid droplet and lower expression o（ PPARγ/2 mRNA. Conclusions These data suggest proper mechanical strain could improve the effect on the differentiation of MSCs to osteoblasts, while inhibit the effect on the differentiation of MSCs to adipocytes.

关 键 词：牵张应力 间质干细胞 细胞分化 

分 类 号：R686[医药卫生—骨科学]