大河猪76个STR基因座的遗传多态性  

Genetic Diversity of 76 STR Loci in the Dahe Pig

作　　者：霍金龙[1] 霍海龙[1] 苗永旺[1,2] 李福泉[3] 刘丽仙[4] 伍革民[1] 欧阳依娜[1] 钱坤[5] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南大学生物资源保护与利用国家重点实验室,云南昆明650091 [3]内江职业技术学院生物技术系,四川内江641100 [4]云南农业职业技术学院畜牧兽医系,云南昆明650212 [5]湖州师范学院,湖州313000

出　　处：《Zoological Research》2009年第1期105-108,共4页动物学研究（英文）

基　　金：云南省教育厅科学研究基金项目(07Y40124);国家自然科学基金项目(30660024);云南省应用基础研究重点项目(2007C0003Z);云南省应用基础研究计划面上项目(2006C0034M)

摘　　要：大河猪是中国西南中海拔地区代表性猪种之一,长期以来在当地养猪生产中发挥了重要作用。为了阐明其群体遗传变异情况,为进一步有效保护和合理利用提供科学依据,采用分布在家猪19对染色体上的76个微卫星标记对该猪种60个随机抽样个体进行了微卫星PCR–聚丙烯酰胺凝胶电泳检测。共检测到347个等位基因,所有座位都呈现出多态性,每个座位的等位基因数在3—10个之间,平均每个座位等位基因数4.57个,有效等位基因数3.50个,群体平均杂合度及平均多态信息含量分别为0.6962±0.0716和0.6441±0.0914。结果表明,大河猪群体遗传多样性较丰富,选择潜力较大。The Dahe pig is one of the representative indigenous pig breeds in the middle-altitude region of southwest China, and has played an important role in the local meat production for a long time. In order to estimate the genetic variability in its population, as well as provide the guidance and basis of genetic background for its conservation and further utilization, 76 microsatellite loci situated on swine＇s 19 chromosomes were analyzed for 60 individuals sampled from their natural population by employing PCR and polyacrylamide gel electrophoresis. A total of 347 alleles were detected and all the loci were polymorphic. The number of alleles varied from 3 to 10, with a mean allele and effective allele number of 4.57 and 3.50 at each locus respectively. The mean heterozygosity and polymorphism information content of 76 microsatellite loci were 0.696 2±0.071 6 and 0.644 1±0.091 4, respectively. The results indicated that the genetic diversity of the Dahe pig was rich, and the Dahe pig has a good selection potential for breeding.

关 键 词：微卫星标记 大河猪 遗传多样性 杂合度 多态信息含量 

分 类 号：S828[农业科学—畜牧学] Q953[农业科学—畜牧兽医]