硼中子俘获疗法诱导U87胶质瘤细胞凋亡  

Boron neutron capture therapy inducing apoptosis in U87 glioblastoma cells

作　　者：王鹏[1] 章翔[1] 江新标[2] 程光[1] 赵英[2] 曹锐峰[1] 刘红[3] 王剑博[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,西安710032 [2]西北核技术研究所 [3]第四军医大学校务部卫生处

出　　处：《中华神经外科杂志》2009年第1期57-60,共4页Chinese Journal of Neurosurgery

基　　金：基金项目：国家自然科学基金（10375047）

摘　　要：目的探讨硼中子俘获疗法（BNCT）对人脑胶质瘤细胞株U87的增殖抑制和诱导凋亡的作用及可能机制。方法实验分为未照射组（0Gy）、γ射线对照组（4、8Gy）、反应堆组（3．5Gy）、BNCT组（4、8Gy）。采用形态观察、流式细胞仪Annexin V／PI荧光染色、四甲基偶氮唑蓝（MTT）法等方法观察BNCT对U87细胞的增殖抑制和诱导凋亡的作用，以免疫组织化学技术检测P53蛋白的表达，应用western blot检测BCL-2、BAX蛋白表达的变化。结果硼中子照射后细胞出现典型的凋亡形态改变。BNCT4、8Gy组处理后48h细胞的凋亡率分别为65．1％、85．9％。BNCT4、8Gy组细胞生长抑制作用显著高于同等剂量的吖射线照射组（P〈0．01）。未照射的U87细胞P53蛋白表达阴性，BNCT4、8Gy照射后P53蛋白表达阳性。BNCT4、8Gy照射后BCL-2蛋白表达下降，BAX蛋白上升。结论BNCT对U87细胞具有显著的增殖抑制作用，并有剂量、时间依赖性特点。Objective To explore the effect of boron neutron capture therapy （BNCT） on human brain glioma U87 cell line and its mechanisms. Method U87 cells in exponential phase were divided into 6 groups： untreated control,^60Co γ 4 Gy,60 Co γ 8 Gy, nuclear reactor exposure without boronophenylalanine （BPA） 3.5 Gy, BNCT 4 Gy and BNCT 8 Gy. The anti-tumor effects were analyzed through cell morphology, the Annexin V/PI assay by flow cytometer （FCM）, and methyl thiazolyl tetrazolium （MTF） assay. The expression of P53 protein was studied by immunocytochemistry and the expression of BCL - 2 protein and BAX protein were measured by western blot. Results Typical morphological changes were observed after BNCT irradiation. The apoptotic rates were observed 48 h after irradiation with 65. 1% and 85.9% for BNCT 4 Gy and 8 Gy. BNCT showed higher apoptotic rates than those of γ - ray control irradiation （P〈0.01）. The expression level of P53 protein was negative in U87, while positive expression of P53 protein was observed in BNCT 4 Gy and 8 Gy groups. BNCT promoted BAX protein expression,at the same time it also inhibited BCL-2 expression. Conclusions BNCT can inhibit the growth of U87 significantly in a dose-dependent and time-dependent patterns.

关 键 词：硼中子俘获疗法 神经胶质瘤 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]