氧化亚铁硫杆菌中磁小体形成相关基因在不同亚铁浓度刺激下的差异表达  

Response of genes for synthesizing the magnetic of Acidithiobacillus ferrooxidans to different concentration of Fe^(2+) stress

作　　者：刘新星[1] 武海艳[1] 刘文斌[1] 李寿朋[1] 

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083

出　　处：《生物工程学报》2009年第1期69-75,共7页Chinese Journal of Biotechnology

基　　金：国家自然科学基金项目(No.50774102);国家重点基础研究发展规划项目(No.2004CB619201);中南大学研究生教育创新工程(No.1343-77341)资助~~

摘　　要：氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)的生物控制矿化作用可以使其在胞内形成黑色电子致密颗粒—磁小体。本研究利用生物信息学方法对氧化亚铁硫杆菌标准菌株ATCC23270的全基因组进行分析,并通过Real-timePCR技术研究氧化亚铁硫杆菌中与磁小体形成相关的mpsA、magA、thy和mamB四个基因在不同亚铁浓度刺激下的差异表达,结果发现它们在转录层面的表达量受亚铁浓度的影响,当亚铁浓度达到150～200mmol/L范围内达到最高表达,这对进一步深入研究氧化亚铁硫杆菌中磁小体的形成机理有积极的意义。Acidithiobacillus ferrooxidans is able to synthesize intra-cellular electron-dense magnetite, which formed by BCM method in Acidithiobacillus ferrooxidans. The whole genome of the type strain Acidithiobacillus ferrooxidans ATCC 23270 was analyzed by bioinformatics and some homolog genes of functional ones in magnetotactic bacteria were available. This study analyzed the different concentration of Fe^2＋ stress response of mpsA, magA, thy and mamB gene by using real-time PCR analysis. Temporal genes expression profiles were examined in cells subjected to different concentration of FeSO4·7H2O stress, they reached to high expression under 150-200 mmol/L FeSO4·7H2O stress. With this new method study, it is possible that we could do deeper research to generate a comprehensive description of the mechanism that how Acidithiobacillusferrooxidans synthesize the magnetic particles.

关 键 词：氧化亚铁硫杆菌 磁小体 生物信息学 实时定量PCR 

分 类 号：Q93[生物学—微生物学]