预处理方式对微紫青霉菌吸附Cu^(2+)的影响及其吸附机理  

Effects of pre-treatment on Cu^(2+) absorption of Penicillium janthinellum strain GXCR

作　　者：黄晓婷[1,2,3] 孙长斌[1,2,3] 陈小玲[1,2,3] 秦会娟[1,2,3] 胡美[1] 袁媛[1] 李有志[1,2,3] 

机构地区：[1]广西大学生命科学与技术学院,南宁530005 [2]微生物及植物遗传工程教育部重点实验室,南宁530005 [3]广西亚热带生物资源保护利用重点实验室,南宁530005

出　　处：《生物工程学报》2009年第1期76-83,共8页Chinese Journal of Biotechnology

基　　金：广西科技攻关项目(Nos.0895003-8和0443001-20);广西亚热带生物资源保护利用重点实验室主任基金(No.07-04);微生物与植物遗传工程教育部重点实验室开放基金(No.J0701)资助~~

摘　　要：为有效提高青霉菌(Penicillium)对含Cu2+的水溶液中的Cu2+的吸附能力并了解其吸附机制,研究了8种不同预处理方式对微紫青霉菌(Penicillium janthinellum)菌株GXCR的Cu2+吸附的影响。结果表明,匀浆化、匀浆+碱化(NaOH)(简称匀浆碱化)、高温(80oC)、匀浆+盐化(NaCl)、匀浆+洗涤剂和匀浆+极化(二甲基亚砜)可显著提高菌体的吸附率,但是匀浆化+酸化处理会导致菌体的Cu2+吸附能力显著下降。与早期其他研究相比,本研究发现匀浆化碱化(NaOH)相结合的方式能够显著提高菌体的吸附能力。其中匀浆碱化(NaOH,0.5mol/L)处理后菌体的Cu2+的吸附率增加了47.95%;匀浆碱化菌体吸附符合典型的Langmuir方程,表明该菌对吸附Cu2+的吸附可能是以表面吸附为主。吸附-解吸循环4次后,碱化菌体的Cu2+的吸附率仍可达到70.82%。红外光谱分析表明碱化处理主要影响菌体表面分子的-OH、C=O和COOH基团中的C=O,其中与Cu2+结合的主要基团是-OH。GXCR的对Cu2+的吸附可能是主要基于Cu2+与菌体-OH基团结合的化学吸附为主。In order to effectively increase capacity of Cu^2＋ absorption by Penicillium from Cu^2＋-containing aqueous solution and to study the mechanisms of absorption, effects of eight pre-treatment methods on Cu^2＋ absorption of Penicillium janthinellum strain GXCR were compared. The results showed that the efficiency of Cu^2＋ absorption obviously increased through pre-treatment by homogenization, homogenization-basification （NaOH）, oven dry （80℃）, homogenization-salinification （NaC1）, homogenization- detergent and homogenization-polarization （C2H6SO）, but significantly decreased after acidification pretreatment with H2SO4. In comparison with the previous reports, the pretreatment in a homogenization-NaOH way could more efficiently enhance the Cu^2＋ absorption capacity of this fungus. Homogenization-basification （0.5 mol/L NaOH） increased Cu^2＋ biosorption by 47.95%. The Cu^2＋ absorption of the mycelia treated by homogenization-basification followed Langmuir isotherm equation, suggesting a surface absorption process. After four cycles of absorption-desorption, mycelia pretreated by homogenization-alkalization still had 70.82% of Cu2＋ biosorption efficiency. Infrared reflectance analysis indicated that alkalization treatment made marked effects on molecular groups of C-H, C=O, and C=O in COOH on the mycelial surfaces, and -OH was a key Cu^2＋-binding group. It is therefore suggested that the Cu^2＋ absorption by the GXCR is likely to be a chemical absorption process through Cu^2＋ binding with -OH group on the mycelia.

关 键 词：真菌 预处理 CU^2+ 生物吸附 解析 吸附机理 

分 类 号：X703[环境科学与工程—环境工程]